dealing with such small quantities of reagents‚ any small inaccuracy in measurement creates a large difference in actual yield from theoretical yield. Through simple molar calculations‚ using the coefficients in the balanced chemical equation( CaCl2(aq) + 2NaOH(aq) Ca(OH)2 + CaCl2)‚ the limiting reagent could be determined from the volumes of reactants used. The number two to four tests turned out as expected‚ NaOH and CaCl2 respectively being the limiting reagents. In the second test‚ according to
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Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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Question 1 (Limiting Reagent) 15.00 g aluminum sulfide & 10.00 g water react until the limiting reagent is used up. [Atomic mass: H = 1.008‚ Al = 26.98‚ S = 32.07‚ O = 16.00] Here is the balanced equation for the reaction: Al2S3 + 6 H2O ( 2 Al (OH)3 + 3 H2S (i) Which of the two reactants is the limiting reagent? (ii) What is the maximum mass of H2S which can be formed from these reagents? (iii) How much excess reagent remains after the reaction is complete
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Determining the Stoichiometry of Chemical Reactions Objective In this lab we took Fe(NO3)3 and NaOH and mixed 7 different mole ratios in graduated cylinders to determine what the mole ratio is. We also did the same thing with solutions of CuCl2 and Na3PO4. We determine the mole ratios by graphing the volume of reactant #1 vs. volume of precipitate for each reaction. Data Part 1.) Cylinder 1 2 3 4 5 6 7 Fe(NO3)3‚ 0.1 M‚ ml 5 10 12 15 17 20 24 NaOH‚ 0.1 M‚ ml 55 50 48 45
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“Use of Biuret Reagent to Test for Proteins In Food” Cindy Zhou Introduction Protein is a series of amino acids that are connected together by peptide bond. The bond is located between the end of previous amino acid‚ which is the carboxyl group‚ and the head of next amino acid‚ which is a amino group. The Biuret reagent is a solution of copper sulfate and potassium hydroxide. When peptide bond are presented‚ the copper (II) will interact with them (MadSci Network: General Biology). It is
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| Reactions of Grignard Reagents with Carbonyls | | | Tuesday 1:30 | 2/28/2012 | | Introduction This experiment explores the reactivity pattern for the addition of Grignard reagents to three different carbonyl groups: a ketone‚ an ester‚ and a carbonate. Grignard reagents are organometallic compounds that have a carbon-metal bond‚ such as carbon-magnesium. Grignard reagents are formed from the reaction of an alkyl‚ cycloalkyl‚ or aryl halide and magnesium metal in dry ether
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observe the reactions of specific aqueous solutions with specific aqueous reagents. Introduction: A solution is as a homogeneous mixture containing two or more substances. Reagents are added to solutions to create a chemical reaction or added to see if anything occurs. Reagents can be added to solutions to see if there is a presence of other substances. For example‚ iodine added to a lead solution. Iodine would be the reagent and would cause a chemical reaction confirming the presence of lead. We
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Limiting Reagents and Percentage Yield Worksheet 1. Consider the reaction I2O5(g) + 5 CO(g) -------> 5 CO2(g) + I2(g) a) 80.0 grams of iodine(V) oxide‚ I2O5‚ reacts with 28.0 grams of carbon monoxide‚ CO. Determine the mass of iodine I2‚ which could be produced? 80 g I2O5 1 mol I2O5 1 mol I2 1 333.8 g I2O5 1 mol I2O5 28 g CO 1 mol CO 1 mol I2 253.8 g I2 1 28 g CO 5 mol CO 1 mol I2 b) If‚ in the above situation‚ only 0.160 moles‚ of iodine‚ I2 was produced
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higher absorbance level. Results Table No. | Albumin(cm3) | KCl (mL) | Biuret Reagent (mL) | Absorbance | 1 | 0 | 5 | 3 | 0.049 | 2 | 1 | 4 | 3 | 0.112 | 3 | 2 | 3 | 3 | 0.258 | 4 | 4 | 1 | 3 | 0.39 | 5 | 5 | 0 | 3 | 0.418 | 6 | 5 (A) | 0 | 3 | 0.198 | 7 | 5 (B) | 0 | 3 | 0.347 | Table 1) Test Result Table 1 is the results of seven test tubes with a different amount of solutions. 3 mL of Biuret Reagent was added to all seven test tubes and absorbance was measured with spectrophotometer
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figure out who was guilty by determining which macromolecules are present in the mystery food sample. Question Which macromolecules are present in the mystery food sample? Hypothesis If the mystery food is tofu‚ then it will react with the reagent that indicates the presence of a protein because tofu is primarily protein. Variables Independent variable: presence of macromolecules‚ including monosaccharides‚ polysaccharides‚ proteins‚ and lipids Dependent variable: color changes observed
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