All-in-1 is a company that have been selling macronutrient products for the past 6 years‚ and recently they’ve been getting accused of that they have not included the important macronutrients in one of their products; the meal replacement drink. The nutra-chek company‚ takes samples of food products into their labs to test for macronutrients in the samples. The purpose of this lab is to test for four macronutrients: Monosaccharides‚ starches‚ proteins and fats. The results of this lab will determine
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the presence of monosaccharides by using the Benedict’s test Test for the presence of starch‚ a polysaccharide‚ by using the Lugol’s iodine test Test for the presence of lipids by using the Sudan test Test for the presence of proteins by using the Biuret reagent test Determine the identity of an unknown substance by detecting the presence of organic macromolecules using the above tests Introduction Biological macromolecules are defined as large molecules made up of smaller organic molecules. There
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When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a cracker that is positive for starch and negative for a reducing sugar. Iodine would test if starch was present
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Introduction The objective of this experiment is to identify if the 12 provided solutions contain starch‚ glycogen‚ reducing sugars or proteins. The four major biological macromolecules are carbohydrates‚ lipids‚ proteins and nucleic acids. Abundant elements in the world such as carbon and nitrogen bond in different ways to form many different molecules. The functional groups are what determine their characteristics. During the experiment‚ there will be 3 test. The iodine test is completed to
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blue. (+) | 10 drops honey solution | Bright orange‚ an abundance of reducing sugars. | Yellow and clear. (-) | Unknown #4 | Reddish orange‚ an abundance of reducing sugars. | Yellow. (-) | Table 6.2 Solutions and Color Reactions for the Biuret Test for Protein Solution | Color | 2 mL egg albumen | Dark violet (+) | 2 mL honey | Yellow (-) | 2 mL amino acid solution | Pale blue (weak +) | 2 mL distilled water | Pale blue (weak +) | 2 mL protein solution | Violet (+) | Unknown
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form through fused rings‚ their function is to carry cholesterol‚ phytosterol‚ and hormones. The Biuret Reagent test is a straightforward test to identify lipids. To perform this test‚ one must combine equal amounts of a lipids and Biuret Reagent solution‚ let it set for several minutes before analyzing. Once the mixture has settled into its color‚ take a look‚ the substance should have the blue Biuret Reagent solution settled at the bottom and the lipid floating clearly on
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The most common organic molecules are carbohydrates‚ lipids‚ and proteins. Carbohydrates contain carbon‚ hydrogen and oxygen with a ratio of 1:2:1‚ this means that for every 2 hydrogen atoms there is one carbon atom and one oxygen atom. They are necessary for the body to function properly because they provide the fuel that burns to produce energy. Carbohydrates come in the class of either a monosaccharide‚ disaccharide‚ or polysaccharide. Monosaccharides are the simplest form of a carbohydrate‚ disaccharides
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reduced copper in alkaline solutions. This is called a "Biuret" chromophore. The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate) by tyrosine and tryptophan residues. The reduced Folin-Ciocalteu reagent is blue and therefore is detectable with a spectrophotometer in the range of 750 nm. Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone. The BCA Assay: The BCA assay is
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ntroduction The purpose of this experiment was to use Iodine‚ Benedict and Biuret to test the reaction of the following 12 samples: 1% glucose‚ 0.3% glucose-1-phosphate‚ 1% maltose‚ honey‚ 1% sucrose‚ 1%lactose‚ 1% glycogen‚ 1% starch‚ protein‚ beer‚ distilled water and an unknown solution (test tube: 300). The iodine test for starch was to test how would starch reacted if we put iodine in it. The color of starch before the test was clear. The color of the iodine was brown. When you added iodine
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RNA Analysis 1. The color result is light brown. 2. A positive Biuret test indicates the presence of proteins in the filtrate. Biuret is used to test for proteins. It is a light blue color and changes to purple in the presence of protein‚ a polymer made of amino acids. It will not change color when only amino acids are present. 3. What type of molecules present in the suspension is hydrolyzed by the mild acid treatment? 4. The products of hydrolysis are peptones‚ peptides‚ and amino acids
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