When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a piece of bread that is positive for starch and negative for a reducing sugar. Iodine would test if starch
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Test for the Presence of Macromolecules Introduction In this experiment‚ we are trying to identify the presence of macromolecules in certain solutions. As in our daily lives‚ macromolecules are the essential nutrients that we have to take every day‚ therefore we would like to identify the types of macromolecules that contain in some of the food or solution in order to maintain a healthy life. There are four groups of macromolecules which are carbohydrates‚ lipids‚ proteins and
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Bradford Protein Assay Practical Report 1. Present your data (including raw data and calculated concentrations) for the protein standards in the form of a clear table. Give one example of how you calculated protein concentration. Do not forget a descriptive title and units (4marks) Title either too long or not descriptive or absent Your results are in duplicate & shouldn’t be referred to as ‘set1 & set2’ or ‘original’ and ‘duplicate’ Many of you think units of absorbance are nm but A has arbitrary
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starch protein d. Biuret test - glucose tap water oil (lipid)) starch protein 7. All proteins contain carbon‚ hydrogen‚ oxygen and what other element? Nitrogen 8. When one glycerol molecule covalently bonds via dehydration synthesis with three fatty acid molecules the resulting macromolecule is called a __lipid_________. 9. What are the two general categories of carbohydrates? Starch and cellulose ID the test: Sudan IV‚ Benedict’s‚ Biuret‚ Starch 10. The
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only substance who turned shiny afterwards. All of the other ones were matte. Describe the positive test for protein. Explain how you know. The positive test for protein is adding the Biuret solution. I know this because the protein is the substance who had the most dramatic reaction with the Biuret Solution. While all of the other substances turned blue and yellow‚ the protein turned purple. What substances were present in the unknown solution? Explain how you know. The glucose
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molecules. These tests take advantage of the fact that different types of molecules have different functional groups and‚ therefor‚ different chemical properties. Thus‚ they will react to test reagents (also called indicators) differently. Materials: Biuret‚ Sudan III‚ Iodine‚ Benedict‚ water‚ hot plate‚ paper towels‚ pipette‚ ruler‚ goggles‚ and a timer. Method: Benedict’s test for reducing sugar: 1. Ruler was used to mark 1 cm and 2 cm marks on each tubes. 2. Negative control tube was filled with
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record their results. 7. Have students return to their desks but stay with their groups to complete the Analysis Questions in their journals. Alternate indicators: Iodine for lipids/oil (turns pink but transformation is temporary so watch carefully) Biuret Solution is easy to make. Start with 40% NaOH and
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Practical 2: Observation of mammalian kidney model and tissue slides. Analyzing kidney filtration using simple filtration system Introduction: Kidney is part of mammalian’s body endocrine system. Every mammals have a pair of kidneys that is located at the middle back of the body and symmetrically beside the spine and below the rib cage. A kidney approximately 0.5% of the organism body weight. Every kidney will receive huge amount of blood to enable them to perform important task. The base unit of
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Version 42-0085-00-01 Lab Report Assistant ________________ Name: Akil Kelly Exercise 1: Testing for Proteins Data Table 1: Biuret results. Substance Tested Predicted Results Biuret Color & Number of drops added 1: Egg white Contains Protein Purple color – 9 drops 2: Pepsin Contains Protein Purple color – 9 drops 3: Sugar Doesn’t contain protein Blue color – 9 drops 4: Distilled water Doesn’t
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because it is important to know what is in the food you eat for yourself and not always reply on the vague claims that companies make about their products. ● Description: Knowing that sugars react to a Benedict’s reagent‚ proteins will react to a Biuret reagent‚ and starch will react to a Lugol’s reagent‚ we will be able to test the presence
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