Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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! EXERCISE 6! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! BIOLOGICALLY IMPORTANT MOLECULES! REPORT ! ! ! INTRODUCTION ! In this experiment‚ we will learn about biological important molecules by performing tests to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Also‚ we will use biochemical tests to identify the unknown compound that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Sudan IV test‚ Biuret’s
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tube. The Biuret reagent was made by the mixing of two chemicals. 10. The reaction with Biuret reagent was observed and recorded. 11. The solutions were disposed as stated in the 8th procedure; the lab equipment and chemicals were returned to the teacher. CONCLUSIONSThrough this lab‚ we learned how to determine protein‚ lipids‚ glucose and starch with their indicators from unknown samples. During the lab‚ I have noticed that the protein solution reacted most dramatically with the Biuret reagent
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corn oil Oats and water 20 ml gelatin and water solution 20 ml potato and water mixture 20 ml apple juice and water mixture 20 ml unknown substance #1 20 ml unknown substance #2 Paper towels 600 ml beaker Brown paper Biuret reagent Benedict’s solution Iodine solution Procedure: 1. Place test tubes in the test tube rack. Use the grease pencil to label each test tube based on the substance that will be placed in them. 2. To test for starches‚ fill each test
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Determination of the presence of carbohydrates and protein in aqueous solution samples Objectives To determine the presence of starch‚ glycogen‚ reducing sugar‚ peptide‚ and proteins by utilizing Iodine test‚ Benedict test‚ and Biuret test. Introduction The purpose of this experiment was to identify the presence of macromolecules by using various positive and negative controls. The principle building blocks of living organisms are essentially constructed by carbon-containing
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Title: Benedict ’s test for Reducing Sugars Testable Question: Are all carbohydrates reducing sugars? Hypothesis: If the carbohydrate contains available ketone or aldehyde molecules then they are reducing sugars. Variables: a. Independent: Function groups react w/ reagent b. Dependent: Color change (red‚ orange‚ yellow) Control: Water Materials: - 4 mL of each solution (Water‚ Starch‚ Glucose‚ Maltose‚ Sucrose‚ Onion Juice‚ Potato slice‚ Milk) - 8 test tubes
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includes to find the acidity and basicity of the fertilizer with the help of pHsolution. Morever I would also find out the solubility of the fertilizer in universal and organic solvent. To end the analysis I would furthermore test the presence of biuret which is toxic for plants and thus impairs plant growth. PRINCIPLE Qualitative analysis means to analyse cations and anions and other elements present in the given samples. The physical and chemical `properties will be observed for the different
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Who Took Jerell’s iPod? Lab Purpose: Standardized Tests The purpose of testing the organic nutrients (vegetable oil‚ glucose‚ starch‚ powdered egg white) with each of the different indicators (brown paper towel‚ benedicts‚ iodine‚ biurets) was to determine the color it would turn with positive traces of the nutrient. The purpose of testing just the Distilled H2O was to determine the color it would turn with negative traces. Nutrient in the Different Foods The purpose of testing each of the
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amino acids. What atom is present in an amino acid that is not present in a sugar molecule? One nitrogen atom is present per molecule in an amino acid. Data Collection: Sample Before Heating A. Benedict’s Solution +Heat B. Iodine Solution C. Biuret Reagent 1. Protein Solution Purple Light Purple Dark Orange Light Purple 2. Glucose Solution Light Blue Orange-yellow Light Orange Transparent
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Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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