three chemicals‚ Lugol’s solution‚ Benedict’s solution‚ and Biuret solution. The tests were qualitative and tested for the presence of sugars‚ starches‚ or proteins within a given substance. The substances were already known‚ so the tests were used to figure out what each solution showed presence of. After going through all of the tests‚ Lugol’s solution showed the presence of starch‚ Benedict’s solution showed presence of sugar‚ and Biuret solution showed presence of protein. In this lab‚ these solutions
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Title: Testing for the Presence of Starches‚ and Proteins Purpose: To test for the presence of starches and protein macromolecules‚ to introduce the concept of variations in chemical compounds. Hypothesis: If biuret is blue after a test for protein‚ then it is a negative result because biuret reagent is blue to begin with. If a solution is pinkish purple‚ or purple‚ then the test for protein is positive. The test solution for starches is yellowish brown. If any substance that is yellowish brown when
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five different sections; we will be using the Biuret Test for amino groups present in proteins. The Iodine Test to identify the presence of starch. The Grease Spot Test and the Sudan Dye test to identify the presence of lipids (fatty acids). The Benedict’s Test to identify the presence of reducing sugars. In the biuret test if the test result for protein changes a color of lavender‚ purple or pink‚ the test result for protein is positive using the Biuret reagent. In the iodine test If the Iodine turns
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” in Matyas‚ M.L. & Haley-Oliphant‚ A.E. (Editors). (1997). Women Life Scientists: Past‚ Present‚ and Future – Connceting Role Models to the Classroom Curriculum. Bethseda‚ MD: American Physiological Society‚ p.291-306 Gurien‚ M. (09 August 2008) “Biuret Test” Ohio University. 29 September 2010. Karp‚ G. E. (2008). Cell and Molecular Biology. 5th Ed. John Wiley & Sons‚ Inc. New Jersey. pp. 40-50; 74-75. Lea‚ C. (2002) “Biological Macromolecules” University of New Mexico. 29 September 2010. Ophardt
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foods but the few most well-know are the Molisch test‚ Bennedict test and the iodine test. Proteins are made up of amino acids‚ which are the simplest unit of proteins. The few maintests to identify the present of the proteins are Xanthoprotic test‚ Biuret test and also Ninhydrin test.The test used to identify the present of fats are the Grease spot test. This can be carried out by using a piece of filter paper. Although it cannot identify the types of fats present but it can show the present of fats
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obtained a test tube rack and 9 test tubes. We had three unknown solutions‚ A‚ B‚ and C. We filled 3 test tubes with the solution A‚ the next 3 with solution B‚ and the last 3 with solution C. The first set of three test tubes A B C we drops few biuret reagent into it‚ then we gently shake the test tubes‚ and observed the test tubes for color change. In the next set of 3 test tubes A B C we put a few drops of Benedict solution into it and we gently shake the test tubes back and forth and then
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Materials • Samples of different types of milk • Benedict’s Reagent • Biuret Reagent • Sudan III • Water bath • Pipettes/syringes • Test tubes • Microscopic slides and cover slips • Microscope Method (testing for reducing sugars) 1. Add 3cm³ of whole milk‚ by using a pipette or syringe to the test tube. 2. Add 5cm³ of Benedict’s reagent and place it in the boiling water bath for 8 minutes. Do the same for semi-skimmed milk and skimmed milk. 3. Once all 3 of the test tubes are
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To test for proteins‚ one must use biuret reagent. “Biuret regent contains solutions of sodium or potassium and a small amount of dilute copper sulfate solution” pg 28. The reagent then changes color to see if there is a sign of proteins. I think that the distilled water will show a negative result‚ Albumin will show a positive result‚ Pepsin will show a positive result and starch will show a negative result. For this experiment a person would need biuret reagent‚ four test tubes‚ distilled water
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Oil 14. Biuret Reagent 15. Iodine 16. Benedicts Reagent Methods In this lab there are I complete two different tests. The procedure was relatively the same for each test. I would first add 3ml of a given substance to a test tube‚ followed by 5 drops of either Biuret Reagent or Iodine. Once the agent is added‚ I swirled the two substances and then observed the results. The first was to test proteins‚ and the second‚ was to test starches. In the first test‚ Proteins‚ I used biuret reagent to
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In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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