in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature‚ cited over 200‚000 times. The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1.0 mg/mL and is based on the reaction of Cu+‚ produced by the oxidation of peptide bonds‚ with Folin–Ciocalteu reagent (a mixture
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Danny Fish 10/9/11 Chemical Testing To identify An Unknown The hypothesis tested was that depending on the solution presented‚ which would test positive for one of the following‚ proteins‚ carbohydrates‚ or lipids through use of chemical testing. (Sudan IV‚ Benedicts’ Solution‚ Iodine‚ Biuret’s) . In order to gain more information for the hypothesis‚ one must know how to test for said macromolecule. Each of the above stated molecules has their own individual solution that will in turn identify
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Biological Molecules: Carbohydrates‚ Lipids‚ Proteins By the end of this lab‚ the student should be able to: Identify the functional groups for each of the biomolecules that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Brown Bag test‚ Sudan III/IV test‚ and the Biuret’s Test. Describe the mechanism of reaction for: Benedict’s test‚ Iodine test‚ Sudan III/IV test‚ and the Biuret’s Test. Interpret the results when presented with data for each of the biochemical
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What are the central ideas of Cells? • What are cells? – Cells are the smallest basic unit of living things. • What do they do? – Cells take in raw materials and making new substances. • Why do they do this? – In cells‚ organelles carry out activities to keep the organism alive. – E.g. synthesising proteins and fats‚ releasing energy from glucose. What are the central ideas of Movement of Substances? • What processes describe how substances move from one region to another? –
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Unit 1 Lesson 1 Key Question Biology SBI4U-C ILC 1) Mg2+ Has 10 electrons. 2) Carbon -14 would not be effective at dating bones that are millions of years old. Carbon -14 is effective at dating to a maximum of 40 000 to 50 000 years old. The isotope decays over time and would not be present on bones that are millions of years old. 3) Hydrolysis - Water used to breakdown molecules. Hydro means water and lysis means to breakdown. An example of hydrolysis in action within our bodies is how our
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Test Biuret Test Reagents CuSO4 sol’n‚ diluted NaOH Test for Intact proteins and protein hydrlysates (at least tripeptide‚ 2 peptide bonds Result Pink to violet blue color Ninhyrin Test 1‚2‚3-indanetrione monohydrate or triketohydintene hydrate‚ ethanol Alpha- amino group (usually a general test for amino acids) Xanthoproteic Test Conc. HNO3‚ conc. NaOH For W‚F‚Y (aromatic except for H) Blue to blue-violet Oxidative decarboxylation color & deamination
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area‚ type your observations‚ mainly focusing on the color‚ but also the layering as appropriate. Be sure your interpretations match your observations! The chart area will expand as you type. BENEDICT ’S TEST FOR SUGAR IODINE TEST FOR STARCH BIURET TEST FOR PROTEIN SUDAN TEST FOR LIPID WATER (-) - - - - _OBSERVATIONS_ Blue Yellow Light blue Clear w/ red layer on top GLUCOSE (+) + - - - _OBSERVATIONS_ Red-Orange Yellow Light blue Clear w/ red layer on
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ABSTRACT ------------------------------------------------- Food contains nutrients that are very significant to the body. The basic essential nutrients‚ vitamins‚ and minerals play very significant functions in the different metabolic processes. In order for the nutrients to be known‚ several tests were performed to qualitatively analyze nutrients for the different food samples. Reagents were used as indicators to test common food substances for the presence of specific nutrients. Fruit juice
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Separate preparations were made in each part. In analysis of peptide digestion‚ determination of the presence of polypeptide chains and the extent of digestion through the hard-boiled egg white was performed. All of the test tubes were positive for Biuret test which indicates presence of polypeptide chains while for the rennin test‚ curd formations were observed in test tubes 3 and 4 only. In gastric analysis‚ the values for free acidity and total acidity were computed. The free acidity is 20.83 N
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The digestion of milk fats by pancreatic lipase and the digestion of proteins by pepsin and trypsin By Jenny Lam Abstract The purpose of this project was to determine how pancreatic lipase and bile salts digest fats to fatty acids and glycerol and how pepsin and tryspin digest to polypeptides. Litmus solution (pH indicator) was used for the fats to indicate the occurrence of the digestion of fats in experiment A. Egg whites was used as the substrate for the enzymes in experiment B.
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