Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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higher absorbance level. Results Table No. | Albumin(cm3) | KCl (mL) | Biuret Reagent (mL) | Absorbance | 1 | 0 | 5 | 3 | 0.049 | 2 | 1 | 4 | 3 | 0.112 | 3 | 2 | 3 | 3 | 0.258 | 4 | 4 | 1 | 3 | 0.39 | 5 | 5 | 0 | 3 | 0.418 | 6 | 5 (A) | 0 | 3 | 0.198 | 7 | 5 (B) | 0 | 3 | 0.347 | Table 1) Test Result Table 1 is the results of seven test tubes with a different amount of solutions. 3 mL of Biuret Reagent was added to all seven test tubes and absorbance was measured with spectrophotometer
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Lab: Identifying Nutrients Student Guide Prelab Information Purpose Explore the presence of nutrients in food using a laboratory experiment and deductive reasoning. Time Approximately 45 minutes Scenario Your biology teacher found an unknown food sample in the classroom‚ and there are four suspects: students who have different foods for lunch. Student Food Macromolecule Present Zack Tofu Protein Nicole Salad dressing Lipid Christian Jelly sandwich
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Chemical Composition of Cells Testing for Proteins: The independent variable is the biuret reagent. The dependent variable is the solutions being used as follows; distilled water‚ albumin solution‚ and starch solution. If the protein is present‚ then the biuret will change to a violet color. Authors: Eliza Koundry and Jody Cote Eliza was in charge of adding equal amounts of biuret reagent to the distilled water‚ albumin solution‚ and the starch solution. Jody was in charge
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“Use of Biuret Reagent to Test for Proteins In Food” Cindy Zhou Introduction Protein is a series of amino acids that are connected together by peptide bond. The bond is located between the end of previous amino acid‚ which is the carboxyl group‚ and the head of next amino acid‚ which is a amino group. The Biuret reagent is a solution of copper sulfate and potassium hydroxide. When peptide bond are presented‚ the copper (II) will interact with them (MadSci Network: General Biology). It is
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BIURET FOOD TEST FOR PROTEINS molecules consisting of one or more chains of amino acids.Proteins are complex molecules that react differently to many compounds but are also fragile and cannot withstand high temperatures or strong acids and bases without degrading. The Biuret Method‚ or biuret test‚ is used to detect the presence of peptide bonds. It has this name because it reacts positively to the biuret molecule’s peptide bonds. Correctly evaluating the number of peptide bonds is a step towards
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Biuret Test Biuret Test • Biuret Test is a chemical test used to show/detect the presence of peptide bonds‚ which are the basis for the formation of proteins. Explanation In the presence of peptides‚ copper (II) ion forms a violet-colored coordination complexes in an alkaline solution. This test can be used to asses the concentration of protein because peptide bonds occur with the same frequency per amino acid in the peptide. According to Beer-Lambert Law‚ the intensity of the color and hence
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lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes were used to create a set of protein standards. Biuret solution was added to all 22
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4 given samples A‚ B‚ C and D. Research Question: Out of the four given samples which sample consists of protein/ starch/reducing sugar/ disaccharide? What color changes will help to identify the contents of each sample when treated with iodine‚ biuret agent and benedicts solution? Theory and background information: What is a protein?1 Proteins are macromolecules‚ consisting of one or more chains of amino acid residues. Proteins perform a vast array of functions within living organisms‚ including
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up‚ with one set for each of the following sample solutions: water‚ protein‚ starch‚ lipid‚ vitamin C and an unknown sample. 0.5 L of each solution was added to each tube. One drop of each of the three indicator solutions (iodine‚ Sudan III and Biuret & copper sulfate) was added to each sample solution to determine how that sample solution (and that type of macromolecule) would react with each specific indicator. The results were recorded in the data chart below. The identity of the unknown solution
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