application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to decolorization. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative cells. However this method is not suitable for taxanomic purposes.
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tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon
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harvard.edu/node/222 BSP‚ S. (2010). How is EM different from light microscopy? Retrieved April 25‚ 2015‚ from http://bsp.med.harvard.edu/node/222 Tortora‚ G.‚ Funke‚ B.‚ & Case‚ C. (2010). Staining Techniques. Retrieved April 25‚ 2015‚ from http://www.cliffsnotes.com/sciences/biology/microbiology/microscopy/staining-techniques
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Purpose/Question being addressed: The purpose of this experiment is to determine which kind of drinks stain your teeth the most. The drinks that will be tested are tea‚ coke‚ and coffee. B. Research Facts/ Hypothesis: Research shows that the darker the drink is that you drink the yellower your teeth will become. Teeth are naturally white or off-white in color but build- up from foods and drinks such as coffee and tea over
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Identifying the Unknown Bacteria: Bacillus cereus Brittany Moy Coastline Community College Abstract Bacillus cereus is a Gram-positive‚ facultative aerobic‚ spore-forming‚ rod-shaped bacterium that is widely distributed among the environment. Introduction The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria
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Theories of Staining Techniques Staining bacteria with different dyes via staining techniques‚ allows in distinguishing the microorganism from its backgrounds. Also‚ helps in studying different internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties
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Del Mundo‚ Layah Basella rubra as a biological stain Experimental The alugbati extract turned out to be favorable stain for plant nuclei and organelles especially for Hydrilla verticillata and as substitute for crystal violet or safranin in Gram staining. The extract stained the bacteria red-orange and the plant nuclei‚ and the cytoplasm red and light pink‚ respectively. The study is limited to the ability of Basella rubra (alugbati) to be used as a biological stain 2011 Gel-O-Fury- Saavedra‚ Ralph
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Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
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