Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes‚ biological stains and
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this experiment was to identify a specific unknown bacterium that was isolated from a multitude of bacteria in a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that the bacterium is of the bacillus family due to the rod shape. Due to multiple complications with the PCR‚ a new hypothesis couldn’t be formulated. Methods: In this experiment
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LABORATORY EXERCISE 8-A: Preparation of Bacterial Smear and Simple Staining I. INTRODUCTION Bacterial smears are prepared for the purpose of viewing microorganisms under the microscope. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. A bacterial smear is a dried preparation of bacterial cells on a glass slide. Smears may be made from a dry culture
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SCI250 Week 1 Chapter 3 Staining Lab Quiz Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? A. Gram stain B. Schaeffer-Fulton stain C. Acid-fast stain D. Lipidialar stain E. Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content? A. Capsular stain B. Gram stain C. Spore stain D. Negative stain E. Methylene blue 3
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rapid succession to heat fix the smear. Simple Staining = staining cells with a single dye so that they can be more readily observed. Basic Dye = (methylene blue) has positively charged chromophore group. A basic dye will be attracted to any negatively charged substance‚ such as bacterium. Acidic Dye = (eosin) have negatively charged chromophore groups‚ and thus are attracted to positively charged substances. Chromophore Group = chemical staining group. Bacilli = rod- shaped bacterium. Endospore
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and therefore necessary during cellular proliferation. The protein can be found in all phases of the cell cycle but not in resting cells. The Ki67 staining is used to determine growth of certain cell types and is therefore used on hyperproliferative cells such as tumor cells‚ but also in benign hyperproliferative diseases such as psoriasis. The staining reveals the proliferative active keratinocytes which can be found in stratum basale. Sept7-deficient mice receiving IMQ treatment (Fig S6. A) had
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10% cause disease. The three (3) diseases and their causative
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Through processes know as wet mounts and simple stains‚ observing living microorganisms through a compound microscope can generate a clearer understanding of their appearances and movements. Microorganisms are living things that for the most part cannot be seen through the naked eye. They live on and among humans‚ as well as plants‚ animals‚ and all that is a part of the Earth. Harmless as most seem to be to humans‚ microorganisms are an important relevance of study for human interest in varied perplexities
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Week 1 Chapter 3 Staining Lab Quiz SCI/250 SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each) Check or underline the correct answer Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall
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