experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour ‚ while Staphylococcus aureus ( S.aureus ) is a positive gram bacteria which stain purple colour. Materials Bacteria : Escherichia coli ‚ Staphylococcus aureus Crystal Violet Gram’s iodine Absolute alcohol Safranin Methodology As per manual. Discussion Gram staining is a very importance
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MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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As mentioned above‚ bacterial growth rates during the phase of exponential growth‚ under standard nutritional conditions (culture medium‚ temperature‚ pH‚ etc.)‚ define the bacterium’s generation time. Generation times for bacteria vary from about 12 minutes to 24 hours or more. The generation time for E. coli in the laboratory is 15-20 minutes‚ but in the intestinal tract‚ the coliform’s generation time is estimated to be 12-24 hours. For most known bacteria that can be cultured‚ generation times
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inoculation‚ we were able to visibly see the various bacterial colonies of our biofilms. We proceeded to gram-stain a colony from each biofilm‚ in search of colonies of gram-positive bacteria. There are very specific steps that must be taken when gram- staining bacteria; the first step being to collect a colony of bacteria from one of the TSA plates‚ then transfer the colony from the TSA plate to the slide. We used a sterile loop (sterilized by placing the loop in the flame of a Bunsen burner to eliminate
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of the unknown bacterium were taken after 48 hours of incubation at 35° Celsius. Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure
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most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium. Results:
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Activities/Tasks/Responsibilities | Problem(s) Encountered | Action(s) Taken | Personal Reflection | BacteriologyJuly 4- July 31 | Receiving of specimen‚ Logging‚ Processing of specimen‚ Inoculation into media‚ biochemical testing‚ identification of organism‚ gram staining‚ AFB staining‚ releasing of results‚ culture sensitivity testing. | No major problems were encountered during my rotation for Bacteriology. Minor problems like unfamiliarization of SOP’s and other procedures are encountered. | Asking proper procedures
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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