LAB #11A: Quantification of a Mutagen – Standard Ames Test Sarah R. Villarreal Rogers 1130 INTRODUCTION. In the experiment Lab 6A‚ Diet Coke was determined a mutagen by using a test called the Spot-Overlay Ames Test. The Spot-Overlay Test was designed and named after Dr. Bruce Ames‚ a Scientist and professor of Biochemistry and Molecular Biology at an array of colleges (Ames 2012)‚ the test was designed to determine whether or not something is mutagenic. After the Spot-Overlay Ames Test
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The Human Medicines Regulations 2012 PART 1- Contains provisions and an explanation of the role of ministers and licensing authorities PART 2- How different advisory bodies operate (including the commission on Human Medicines and the British Pharmacopoeia Commission PART 3- Rules for manufacturing‚ importing and wholesale dealing (requiring a legally obtained license) PART 4- Requirement for authorisation to sell/supply medicinal products‚ i.e. they must be from an appropriate marketing authorisation
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The Isolation‚ purification and identification of Proteins assayed From Bovine Liver Using SDS Gel Electrophoresis‚ Mass Spectroscopy and Western Blotting Abstract The purpose of the experiment was to isolate and recognize varying protein solubilization and assaying methods by using bovine liver protein. The experiment implicated the impact of different types of solvents like ethanol‚ water‚ PBS‚ PBS+1% Triton x-100‚ and PBS+2% SDS on protein solubilization. Bradford and Ghosh/Dumbroff methods
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BIOL 1209 Writing Assignment 1 Cover Sheet I certify that the writing in this assignment is my individual work and is my sole intellectual property. It does not contain the ideas‚ or writing of other individuals/authors. Author ___________________________ Date ___________________ Lab Instructor ____________________ Lab Section # ____________ The Change in Allele Frequency of the Male Cichlid Fish Population General Research Question: Lake Malawi’s cichlid
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BIOL 470: Biotechnology Instructor: Dr. Chhandak Basu E-mail: chhandak.basu@csun.edu Phone: (818) 677-4592 Office: 3220B Citrus Hall Office hours: Monday‚ Wednesday‚ Friday 2-3; + Open door policy Please make an appointment before you come to my office: http://www.my.calendars.net/drbasu/ Course objectives: In this course you will learn some of the major concepts in the field of biotechnology and recombinant DNA technology including how microbes‚ plants and animals could be used to produce economically
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Unit 1 – Introduction to the Cell Robert Hooke – built the first microscope (30x magnification); viewed slices of cork called cellula (little rooms). Antoni Van Leeuwenhoek - worked with glass huge improvement in quality of lenses nearly 300x magnification became possible first to observe: * single-celled organisms “animalcules” * protists from pond water * bacteria from his mouth – “father of microbiology” * blood cells * banded pattern in muscle cells * sperm from
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How Much Protein Do You Really Need? Why is protein important? Protein is part of an important food group that you will need in order for your body to be able to function properly. As a macronutrient‚ protein allows your body to undergo proper growth and development‚ as well as to strengthen its immunity against various sicknesses and diseases. Protein is also responsible for acting as the main building block that repairs your tissues‚ organs‚ tendons‚ muscles‚ and even your bones‚ skin‚ and eyes
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Question 5: The chromophore in this assay is Coomassie Brilliant Blue dye. Question 6: It is important to set up a blank to separate the solute (saline) from the protein (stock). By subtracting the absorbance of the blank (which has no protein present) from the original absorbance the absorbance of the protein at each concentration will remain. Question 7: The Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds‚ forming reduced
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Protein Synthesis Worksheet 1. 2. a. Protein synthesis began in the nucleus. b. Chromatin is a diffuse mass located in the nucleus where genetic material is found. c. A gene is a sequence of nucleotides‚ it is important because they form the template and these templates are used for synthesizing proteins. 3. a. Transcription is the first step of protein synthesis it uses a sequence of DNA nucleotides to produce a sequence of RNA nucleotide. b. RNA polymerase bonds to RNA nucleotides together
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Why are proteins important? Proteins can easily be addressed as one of the most important molecules in animal cells. There are many varieties of protein molecules which allow a vast range of biological activities in the cell.( Williams‚ G.(2000))’Proteins are big compound with large molecular masses’’(Williams‚ G.(2000)) whose main chemical constituents are hydrogen‚ carbon ‚oxygen and nitrogen. A protein structure consists of a specific sequence of amino acids called the
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