BIOL 1F90 Expt. #1: Protein Quantification Student Name: Carlos Osorio Student ID: 5220710 Lab Section: 34 Date Experiment Performed: Sept. 26th‚ 2012 Lab Partners: K. Cloutier J. Yang ABSTRACT Protein concentration analysis is primarily done through an accepted form commonly referred to as the Bradford Protein Assay. The main purpose of this experiment was to observe and record the various protein samples’ absorbency values through the calibrated readings of a spectrophotometer
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Experiment 2 Quantification of Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A
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We successfully adapted a commercially available protein detection assay for the quantiWcation of PEI. All experiments described below were performed with linear 25-kDa PEI (Polysciences‚ Warrington‚ PA‚ USA) that we routinely use for the transfection of mammalian cells [4]‚ but it was possible to quantitate other PEIs (diVerent molecular weights; branched) with this method (data not shown). The mixing of a PEI solution with the Advanced Protein Assay (APA) reagent (Cytoskeleton‚ Denver‚ CO‚ USA)
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Memorandum To: NMED 1117 Basic Venipuncture for Allied Health Professionals students From: Victoria Banham Program Assistant BCIT School of Health Sciences 604-432-8727 Toll-free: 1-800-663-6542‚ local 8727 E-mail: victoria_banham@bcit.ca Re: NMED 1117 Basic Venipuncture Term Workshop Information Please check with your instructor for exact date‚ time will be 9am – 2pm. The workshop will be held at the BCIT Burnaby Campus in room 404/406‚ Building SE12. The Campus is
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scientific method(s) to social debate. To Postman’s claim that; while scientists use math to help uncover and describe the nature of nature‚ sociologists use quantification to give precision to their ideas‚ Kornblum would more than likely argue that Postman is essentially describing the same thing and that what he calls “quantification” is a lot more involved than
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Name: Mohammed Alghamdi BIOL lab II 1-A cross gives a 3:1 phenotypic ratio. What are the genotypes of the parents? 3:1. (heterozygous parents) 2- According to Mendel’s law of segregation‚ parents who both have the genotype Aa would produce what gametes? A germ cell Aa undergoes meiosis 3-What is the genotype of a plant that produces green peas? Yellow peas are dominant. Yy or yy when crossed with a heterozygote (Yy) will give some offspring that are green 4- To determine whether an animal with
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metabolism and growth‚ and reproduction. The combination of BIOL 273 and BIOL 373 covers all the major physiological systems. Assumed Background: The course is designed for students with a basic understanding of the structures of the biologically relevant macromolecules and cell biology (as provided by the BIOL 130 prerequisite). This material will not be reviewed in class. Fundamental concepts of cell-cell communication and homeostasis from BIOL 273 will also be assumed‚ and will be reviewed only briefly
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Protein SCI/241 Protein A protein are organic compounds that made up of amino acids and are the building blocks of the cells in the body. Every cell in the body requires proteins to grow and repair themselves so these proteins are necessary for a healthy body and survival. Having so much protein can hurt your body so when you consume so much can get you sick and the right size of protein can keep you from getting sick. They’re five types of protein in everything that we eat. The largest class
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BIOL 101 September 15‚ 2012 Assignment 1 Chapter 1 2. What is Homeostasis‚ and why is it important? Give some examples that show how systems work together to maintain homeostasis? Homeostasis is a term used to describe an internal environment being in a state of constant conditions‚ such as constant temperature‚ pH‚ and etc. It is important because staying at an equilibrium requires the least amount of energy and provides the perfect environment for desired reactions to occur. If at any given
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Proteins Are the basic materials of every body cell. Body cells are constantly wearing out. As a result‚ they are continuously in need of replacement Of the six nutrients group‚ only proteins can make new cells rebuild tissue. By the age of 4 years‚ body protein content reaches the adult level of about 18% of body weight. An adequate supply of proteins in the daily diet is essential for the maintenance of health. The word protein is a Greek derivation and means “of first importance”. Composition
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