BIOL 1F90 Expt. #1: Protein Quantification Student Name: Carlos Osorio Student ID: 5220710 Lab Section: 34 Date Experiment Performed: Sept. 26th‚ 2012 Lab Partners: K. Cloutier J. Yang ABSTRACT Protein concentration analysis is primarily done through an accepted form commonly referred to as the Bradford Protein Assay. The main purpose of this experiment was to observe and record the various protein samples’ absorbency values through the calibrated readings of a spectrophotometer
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Experiment 2 Quantification of Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A
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Lausanne‚ Laboratory of Cellular Biotechnology‚ Lausanne VD1015‚ Switzerland Received 29 April 2004 Available online 28 August 2004 Since the Wrst description of polyethylenimine (PEI)1 as a gene delivery vehicle‚ there has been a strong interest in its use for in vivo and in vitro transfections of mammalian cells [1]. The mechanism of gene transfer by PEI is still unclear‚ and little is known about the formation of PEI– DNA nanoparticles. Such questions are diYcult to address because a rapid and
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DATE: 11/10/12 COURSE CODE: BIOL 2365 Comparative Biochemistry TITLE: Proteins and Amino Acids RESULTS: Table 1: The results of experiment 1; the Lowry Test Volume of Standard Protein/ Unknown (mL) Absorbance at 750 nm 0 0.000 0.1 0.017 0.3 0.135 0.3 0.155 0.5 0.230 0.7 0.323 0.7 0.310 1.0 0.457 1.0 Unknown 1a 0.463 1.0 Unknown 1b 0.433 1.0 Unknown 2a 0.237 1.0 Unknown 2b 0.159 Table 2: The results of Experiment 2; Ninhydrin Test Amino acid Color X
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Test Biuret Test Reagents CuSO4 sol’n‚ diluted NaOH Test for Intact proteins and protein hydrlysates (at least tripeptide‚ 2 peptide bonds Result Pink to violet blue color Ninhyrin Test 1‚2‚3-indanetrione monohydrate or triketohydintene hydrate‚ ethanol Alpha- amino group (usually a general test for amino acids) Xanthoproteic Test Conc. HNO3‚ conc. NaOH For W‚F‚Y (aromatic except for H) Blue to blue-violet Oxidative decarboxylation color & deamination
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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Experiment 1: The Effects of Coal Mining Coal mining‚ particularly surface mining‚ leads to large areas of land being temporarily disturbed. The mine workings collect and conduct water that is in contact with the widespread pyrite‚ a mineral that produces iron and sulfuric acid when exposed to air and water. In this lab‚ you will see first-hand the reasons why mine drainage can be harmful to the local drainage system if left untreated. Materials: (3) 100 mL Beakers 1 tsp. Crushed pyrite 1 tsp. Activated
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Experiment 1 Cyanobacteria Oxygen Production Through Photosynthesis Date Performed: September 2‚ 2014 I. Introduction a. Background Cyanobacteria or blue green algae are renowned for their tolerability and susceptibility even in wide range of environmental conditions‚ a characteristic of many primitive organisms. CNB are believed to be the agents of autotrophic origin of life thus they probably represent the survivors of the earliest photosynthetic plants‚ along with photosynthetic and chemosynthetic
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BIOL 1209 Writing Assignment 1 Cover Sheet I certify that the writing in this assignment is my individual work and is my sole intellectual property. It does not contain the ideas‚ or writing of other individuals/authors. Author ___________________________ Date ___________________ Lab Instructor ____________________ Lab Section # ____________ General Research Question: How does the change in the alleles affect the pigment in the alligators; which also affects
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Experiment 1: Error‚ Uncertainties and Measurements Laboratory Report Jan Luke Mendoza‚ Alexis Vienne Munar‚ Paula Murakami‚ Giorla Joanne Negre Department of Math and Physics College of Science‚ University of Santo Tomas Espana‚ Manila Abstract Throughout the experiment the main goal is to find out about the realities in taking measurements‚ that is‚ that there will always be an uncertainty for each acquired value. And to find out and recognize these uncertainties was handled in the
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