Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
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we first need to know what gram-negative and gram-positive bacteria are and what it’s composed of. Gram-positive bacteria take up the class of bacteria that stain crystal violet in the method of bacterial differentiation. Where gram-negative bacteria are a class of bacteria that does not retain crystal violet. Instead they are counter-stained pink by Safranin‚ and because of this‚ identifying positive is possible. One of the many differences between gram-negative and gram-positive bacteria is their
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are
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February 2011 Vol 55 Chief Editor : Neeta Prasad Joint Director (Prod) : J.K. Chandra Editor : Manogyan R. Pal Cover Design : Manjula Das E-mail (Editorial) : editoryojana@hotmail.com : yojanace@gmail.com (Circulation) : pdjucir_ jcm@yahoo.co.in Website : www.yojana.gov.in Let noble thoughts come to us from every side Rig Veda CONTENTS Grassroots Level Democracy in India : An Assessment...........................................................................5 Mathew
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World conveyed in the film Billy Elliot? ‘Into the World’ describes a concept of a growth and change experienced by an individual – whether the change is physically‚ emotionally or intellectually- from the transition between one stage to another. In this transition there are often barriers and obstacles which the individual faces and needs to overcome. The notion of ‘into the world’ is clearly displayed in the film Billy Elliot (2000) directed by Stephen Daldry. The film Billy Elliot set in Durham‚
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Billy Elliot Traditional ideas about stereotypical gender roles can be challenged or supported with different characters and environments. The visual text Billy Elliot was created in 2000 and directed by Stephen Daldry. The film is about an 11 year old boy (Billy Elliot) who tries to pursue a career in Ballet despite negative stereotyping. Billy must overcome many obstacles in his path including his stereotypical father and brother who are convinced that men cannot do Ballet. Billy Elliot demonstrates
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Alexandria Cooker English 102 M “Billy Budd” Annotated Bibliography 4 March 2013 AMERICAN LITERATURE‚ June 1997; Copyright 1997 by H. Bruce Franklin In this article posted on a Rutgers University page‚ it is discussed that the story of “Billy Budd” written by Herman Melville‚ subject is “capitol punishment and its history.” Throughout the nineteenth century capitol punishment was even an even more cruel and unusual punishment. Many argue that the capitol punishment was for people in power
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