In this lab‚ we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid‚ which is relatively small‚ circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism‚ it is absorbed into the organism’s own genetic code. After this occurs‚ the newly introduced gene begins coding for proteins‚
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BACTERIAL TRANSFORMATIONS USING PVIB II. INTRODUCTION Transformation is the manipulation of a bacterial cell’s DNA in order to alter the cell’s genotype or phenotype by absorbing free DNA from its surroundings. In this lab‚ pVIB plasmid will be used. A plasmid is a segment of DNA that can incorporate itself into the bacterial DNA. Although is not required for growth of the bacterial cell‚ plasmids can provide advantages in stressful environments such as the ability to adapt as environmental
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Name Lab‚ Week # 3 Experiment : BACTERIAL GROWTH AND CONTROLLING BACTERIAL GROWTH Introduction <Include purpose of lab experiment> <brief summary of topic investigating and case studies > <state major finding> Procedure < Include information that the reader would need to repeat your experimental procedure. Do not include any observations or results in this section> Observations and Results Part I: Bacterial Growth Result + or - Appearance after Incubation Gram Stain N/A OF Glucose Broth
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Spontaneous bacterial peritonitis: an acute bacterial infection of ascites fluid. Although a bacterial infection the infecting agent is not easily identified. Spontaneous bacterial peritonitis is commonly seen in patients receiving peritoneal dialysis due to contamination of dialysate. Signs and symptoms: a wide range of symptoms including diarrhea‚ worsening encephalopathy‚ ascites that do not improve following administration of diuretic medication‚ worsening or new renal failure and ileus
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Transformation is the genetic alteration of a cell‚ resulting from the intake of exogenous genetic material through the surrounding cell membrane. The purpose of this lab was to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation of E. Coli
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Transformation of Bacterial Cells with Plasmid DNA Introduction: Transformation refers to the process in which the cell integrates foreign DNA to its genetic code‚ meaning it takes the genes and incorporates them into the cell’s current DNA. Cells that can do this naturally‚ most commonly bacteria and archea‚ are known as competent. The bacteria E. coli do not have high transformation competence under normal conditions‚ but can be manipulated to produce better results using
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Study of Bacterial Growth and Resistance Level to Certain Antibiotics INTRODUCTION Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as
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