Glowing Transformations Abstract In this experiment‚ the idea is to become familiar with the transformation of cells. A well thought out procedure‚ involving a heat shock procedure‚ a good antibiotic‚ an inducer known as arabinose to show the newly expressed DNA by a visible fluorescent glow‚ and a stable control group is what contributes to this experiments thoroughness. It is predicted that the four agar plates will all yield different forms of growth‚ with different coloration and colony
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Transformation is the genetic alteration of a cell‚ resulting from the intake of exogenous genetic material through the surrounding cell membrane. The purpose of this lab was to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked
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Bacterial Morphology MLT1 Task 11- Lab 2 Johnny Archuleta Western Governors University Question A and C answers. A wet mount stain is when a drop of water is placed onto the microscope slide. The water on the slide helps to support the organism and sample. The water fills the space between the cover slip and the slide. This action allows the light from the microscope to pass through the slide and the sample for better visualization of the organisms. A direct stain occurs when a charged
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E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation of E. Coli
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STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
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The disease I’ve chosen to write my research paper on is Bacterial Vaginosis. Bacterial Vaginosis is vaginal condition that can produce vaginal discharge and results from an overgrowth of normal bacteria in the vagina. In the past‚ the condition was called Gardnerella vaginitis‚ after the bacteria that was believed to have caused the condition. However‚ the newer name‚ Bacterial Vaginosis‚ reflects the fact that there are a number of species of bacteria that naturally live in the vaginal area and
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Bacterial are very diverse and quite adaptable‚ but they still need certain factors in order to thrive. The physical requirements for bacterial growth include pH range‚ temperature‚ oxygen concentrations‚ moisture levels‚ hydrostatic pressure‚ osmotic pressure‚ and radiation levels. When considering the pH level‚ bacteria are classified as either acidophiles (acid-loving)‚ neutrophiles (neutral ph range)‚ or alkaliphiles (alkali-loving). The one that causes disease in humans would be the neutrophiles
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Bacterial Biology █ BRIAN D. HOYLE An understanding of the fundamentals of bacterial biology is critical to bacteriologists and other forensic investigators attempting to identify potential biogenic pathogens that may be exploited as agents in biological warfare or by bioterrorists. Fundamentals of Bacterial Biology Bacteria are one-celled prokaryotic organisms that lack a true nucleus (i.e.‚ a nucleus defined by a membrane). Bacteria maintain their genetic material‚ deoxyribonucleic
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Bacterial diseases: Introduction Bacterial diseases include any type of illness or disease caused by bacteria‚ a type of microbe. Microbes are tiny organisms that cannot be seen without a microscope and include viruses‚ fungi‚ and some parasites as well as bacteria. The vast majority of bacteria do not cause disease‚ and many bacteria are actually helpful and even necessary to good health. Millions of bacteria normally live on the skin and in the intestines and can also be found on the genitalia
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Bacterial Evolution In the late 19th century‚ diseases led researchers to find treatments to kill the growth of microbes. The outcome of the search was a new kind of medicine which is known as antibiotics. Antibiotics is a variety of substances that control the growth or kill bacteria. Since bacteria is evolving rapidly and resisting antibiotics due to the overuse of the medicine‚ reducing the need and use would help slow down the bacteria from evolving. There are many things that can be done
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