Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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Fighting Bacterial Growth The purpose of this lab was to determine the effectiveness of antiseptics‚ disinfectants‚ and antibiotics on bacteria. The hypothesis was that if bleach was used‚ it would be the most effective because bleach is commonly used to clean and disinfect various things. The variables that were tested were antibacterial soap and Scope mouthwash for the antiseptics; bleach and ammonia for the disinfectant; and Cipro‚ erythromycin‚ and tetracycline for the antibiotics. All of these
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Bacterial Fermentation Secondary article Article Contents Volker Mu¨ller‚ Ludwig-Maximilians-Universita¨t Mu¨nchen‚ Munich‚ Germany . Introduction Under anaerobic conditions‚ in the dark and in the absence of electron acceptors‚ organic compounds are catabolized by strictly anaerobic or facultatively anaerobic bacteria by internally balanced oxidation–reduction reactions‚ a process called fermentation. In fermentation‚ the organic compound serves as both electron donor and acceptor‚ and adenosine
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immediate threat to human health. [1] In this article we explore contamination from this particular strain of E. coli bacteria (O157:H7) and enteric viruses‚ their potential threats and how to test the water for such contamination. Bacterial Contamination Bacterial contamination of water is usually in the form of coliform bacteria. Coliform bacteria live in soil/vegetation and in the gastrointestinal tract of animals and humans. They thrive in warm‚ wet and dark places.
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Mallory Charland February 9‚ 2015 Mutations of E. coli Lab Report Advanced Biology Deducing Mutations of the Lac Operon of E. coli Abstract: In this lab we determined a possible mutation in unique bacterial strains of E. coli by observing the proper responses of wild type E. coli Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes‚ each containing a different substance (lactose‚ glucose‚ water and lactose and glucose)
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spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution‚we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth curve. INTRODUCTION In this experiment‚we learnt cell growth.Cell growth means increase in the quantity of cellular constituents.Cells in culture double its number because they produce
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veterinarian immediately. Contagious bovine pleuropneumonia is not found in the United States. Suspicion of disease requires immediate attention. Animals are exposed to the CBPP bacteria by aerosol from the cough of infected cattle. Once inhaled‚ the bacterial infects the lungs of the animal. Another route of spread is by direct contact with
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Cited: Michael P. Doyle‚ "Food-borne disease"‚ in AccessScience@McGraw-Hill‚ http://www.accessscience.com‚ DOI 10.1036/1097-8542.YB000610‚ last modified: December 13‚ 2000. M. R. J. Salton‚ et al.‚ "Bacterial physiology and metabolism"‚ in AccessScience@McGraw-Hill‚ http://www.accessscience.com‚ DOI 10.1036/1097-8542.069000‚ last modified: June 10‚ 2004. Robert E. Hungate‚ et al.‚ "Bacteria"‚ in AccessScience@McGraw-Hill‚ http://www.accessscience.com
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