Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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Bacterial Morphology Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation There was several amoeba shaped cells that varied in size. There were five somewhat darker areas that were circular in shape. There were also three large
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Study of Bacterial Growth and Resistance Level to Certain Antibiotics INTRODUCTION Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as
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Morphology ‘If language was just a random collection of words‚ you couldn’t acquire it‚ you couldn’t learn it and you’d be imprisoned in the here and now because you couldn’t talk about what was‚ what might be and what will be…’ You couldn’t construct complete and coherent texts….you’d be in a ‘me Tarzan – you Jane’ situation‚ swinging from the wordtrees‚ pointing at things with little labels on them to try and make your partner understand.’ The myths of grammar (Crystal 2004)
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MORPHOLOGY Ampalaya is a climbing vine‚ nearly or quite smooth‚ annual vine. Tendrils are simple‚ up to 20 centimeters long. Leaves are 2.5 to 10 centimeters in diameter‚ cut nearly to the base into 5 to 7 lobes‚ oblong-ovate‚ variously toothed‚ and heart-shaped at the base. Male flower is about 12 millimeters long‚ and is peduncle‚ with a rounded‚ green‚ and about 1 centimeter long bract approximately at the middle. Female flower is yellow flower‚ about 15 millimeters long‚ long-stalked with pair
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MBK – Lab Report Name: Katie Nave Bacterial Morphology Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms : Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes
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The identification of an unknown bacterial environmental isolate through a series of morphological‚ physiological and differential exercises. The purpose of the environmental isolate report is to learn what is necessary in order to take an unknown environmental isolate (EI) and identify it. This was achieved through a series of exercises that provided information on the morphological‚ physiological and biochemical traits of the EI which were then compiled and interpreted in order to make a presumptive
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Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
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