Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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Bacteria Growth Requirements Microbiology Life as we now it has ended. What is left you ask? Well it is said the only thing that could survive an incident that could end our known way of life is a roach and a pack or Twinkies. In truth the great survivor would be microorganisms. Microorganisms can survive where most cannot due to their size‚ nutritional needs‚ energy requirements‚ and are very good at adapting to different environments (Black 2008). Microorganisms require two things to
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a comparative study on growth rate and lipid content of various microalgae samples collected from biologically and geographically diversified areas on Nepal. Comparisons were also made on samples with same genus isolated from geographically diversified areas (i.e. high altitude (>4000 m) and mid hill (>1000 m))‚ and effect of seasonal variation (with respect to temperature) on similar species of microalgae. Euglena sp.‚ Chlorella vulgaris‚ and Scenedesmus sp. with growth rate of 0.401 day-1‚ 0
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Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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1900s to regulate the growth in batch culture of Saccharomyces cerevisiae18. Yeast producers observed that in the presence of high concentrations of malt‚ a by-product - ethanol - was produced‚ while in low concentrations of malt‚ the yeast growth was restricted. The problem was then solved by a controlled feeding regime‚ so that yeast growth remained substrate limited13. The concept was then extended to the production of other products‚ such as some enzymes‚ antibiotics‚ growth hormones‚ microbial
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Abstract In this experiment‚ Escherichia coli is used as a sample to study the growth kinetic of microorganism in shake flask. A different volume of E.coli was transferred into 250ml Erlenmeyer/shake flask containing media for the nutrient of microorganism. The different volume of microorganism transferred will give the different effect of reading on the constant volume of media used. There are three ways to test the growth kinetic rate of microorganism on shake flask‚ which are by optical density (OD)
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2. Why Choose Microalgae culture or Microalgae as an Ideal biomass? Or Microalgae: advantages and disadvantages The microalgae culture have the infinite potential to be used as primary producers of biomass in coming years‚ eventually they will turn out to be as most significant energy source. They can act as feedstock for several types of renewable fuels such as ethanol‚ methanol‚ biodiesel‚ methane and hydrogen. Table 1 represents certain feedstocks used for biofuel production (Chisti 2007; Dominguez-Faus
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The Effects of Antibiotics on Bacterial Growth Biology II 1996 Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic‚ measuring 1 micron in length. However‚ colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. There are many divisions and classifications of bacteria that assist in identifying them. The first two types of bacteria are archaebacteria and eubacteria. Both groups have common ancestors dating to more
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Bacterial Diversity Project John FreesackSection A24 Kim Daffer‚ John Chang September 23‚ 2012 Introduction: Bacteria are everywhere. Some can be seen with the naked eye and some require a microscope but how do we distinguish one kind of bacteria from another? To answer this question‚ we were required to complete three bacterial labs that helped us to understand what microorganisms are and how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms
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MICROBIOLOGY COURSEWORK The effect of antibiotic concentration on bacterial growth Introduction Null Hypothesis- Increasing the antibiotic concentration has no effect on bacterial growth. Experimental Hypothesis- Increasing the antibiotic concentration decreases bacterial growth. Scientific Theory Bacteria are prokaryotes and can be identified by their shape. Spherical bacteria are called cocci‚ rod shaped bacteria are called bacilli‚ spiral shaped bacteria are called spirilla and
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