to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked‚ and then a small amount of the contents were extracted and placed into two petri dishes containing ampicillin (hinders growth of bacteria). After a couple of days‚ the
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Bacteria Bacteria are microscopic organisms whose single cells have neither a membrane-enclosed nucleus nor other membrane-enclosed organelles like mitochondria and chloroplasts. Another group of microbes‚ the archaea‚ meet these criteria but are very different from the bacteria in other ways. In fact‚ there is considerable evidence that you are more closely related to the archaea than they are to the bacteria! Bacteria are living things that are neither plants nor animals‚ but belong to a group
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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as well as a Gram(-) from the species that were given and the experiment proceeded as follows. METHODS The two streak plates made from the mixture of unknown bacteria showed two different colony morphologies. Colonies of Bacteria A were translucent in color‚ circular in form‚ with entire margins and a smooth surface. Colonies of Bacteria B were cream colored‚ irregular in form‚ with undulate margins and an umbonate surface. Two different morphologies allowed Gram stains to be performed on each of
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the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown under microscopic examination. These microorganisms
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several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter they will use the sugars to make ATP. If the bacteria is a fermenter of lactose/sucrose the EMB
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Prokaryotes are single-celled organisms that can survive in extreme environments. Bacteria is the more numerous type of prokaryotes. The group hypothesizes that the samples taken from different environments will all cultivate diverse morphology in fast growing rates in each environment. The aseptic technique was used to cultivate bacteria from different environments. The diversity of morphology and the growing rate of the bacteria was different in each environment. Introduction Prokaryotes are the oldest
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There are six types of microbes. There is Archaea‚ Bacteria‚ Fungi‚ Protista‚ Viruses and Microbial mergers. Microbes play a big part in people’s health and in the environment. Good Microbes play a big part in people’s health. They mostly play bad parts in health even though the ratio of bacteria in your body is 85% good bacteria and 15% bad bacteria. In World War 1 more people died of illnesses caused by infection than the in actual war. Bacteria multiples really fast – like once every 20 minutes
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stimulating the growth and/or activity of one or a limited number of bacteria in the colon‚ and thus improves host health.” Or “A selectively fermented ingredient that allows specific changes‚ both in the composition and/or activity in the gastrointestinal micro flora that confers benefits upon host well-being and health.” Types: Fructo-oligosaccharides: These are found in fruits like tomatoes‚ banana‚ and kiwi. They feed the good bacteria‚ and promote the absorption of vital vitamins and mineral like
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