1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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Food and Chemical Toxicology 45 (2007) 1650–1661 www.elsevier.com/locate/foodchemtox A comparison of chemical‚ antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils‚ oleoresins and their constituents q Gurdip Singh b a‚* ‚ Sumitra Maurya a‚1 ‚ M.P. deLampasona b‚ Cesar A.N. Catalan b a Chemistry Department‚ DDU Gorakhpur University‚ Gorakhpur 273 009‚ India Instituto de Quimica Organica‚ Universidad Nacional de Tucuman‚ Ayacucho 471‚ S.M. de Tucuman
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estimates indicate that one in six or 48 million people are stricken with a foodborne illness each year‚ resulting in 3000 deaths. Although products such as fruits and vegetables are increasingly being implicated in foodborne illnesses related to Escherichia coli O157:H7 and Salmonella‚ red meat and poultry remain the predominant products contributing to outbreaks and recalls. Accurate and rapid detection of foodborne pathogens is a critical component of the process to reduce risks associated with pathogen-contaminated
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Microbiology Study Guide Chapters 1-6 Chapter 1 Main Themes of Microbiology Microorganisms Most are not Pathogens Prokaryotic-no nucleus or organelles Very simplistic—like bacteria Eukaryotic-has nucleus and organelles like mitochondria Includes fungus‚ and animals Acellular-not even a complete cell Includes viruses and prions like those that cause Mad Cow disease Six main types of microorganisms Bacterium (prokaryotic) Fungus (eukaryotic) Algae (eukaryotic) Virus (acellular)
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Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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References: Bergmans‚ H. E.‚ Van Die‚ I. M.‚ & Hoekstra‚ W. P. (1981). Transformation in Escherichia coli: Stages in the Process Panja‚ S.‚ Aich‚ P.‚ Jana‚ B.‚ & Basu‚ T. (2008). How does plasmid DNA penetrate cell membranes in artificial transformation process of Escherichia coli? Molecular Singh‚ M.‚ Yadav‚ A.‚ Ma‚ X.‚ & Amoah‚ E. (2010). Plasmid DNA Transformation in Escherichia coli: Effect of Heat Shock Temperature‚ Duration‚ and Cold Incubation Wilfinger‚ W. W.‚ Mackey‚ K
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active against most gram-negative bacteria: Pseudomonas aeruginosa‚ Haemophilus influenzae‚ Escherichia coli‚ Shigella spp.‚ Salmonella spp.‚ Neisseria meningitidis‚ Neisseria gonorrhoeae. It is active against Staphylococcus spp. (including strains producing and not producing penicillinase‚ methicillin-resistant strains)‚ some strains of Enterococcus spp.‚ Campylobacter spp.‚ Legionella spp.‚ Mycoplasma spp.‚ Chlamydia spp.‚ Mycobacterium spp. ciprofloxacin is active against bacteria producing
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Castillo Tania Castillo Epidemiology Report of Tuberculosis Tuberculosis (TB) is a chronic bacterial disease that is highly in development countries. This disease is caused by a bacterium called Mycobacterium tuberculosis‚ an acid-fast aerobic bacillus‚ which is prevalent in tropical areas across the globe. For instance‚ the majority of the cases occur in malnourish individuals‚ immigrants and elderly persons. Most of the person’s who has the disease are concurrent for many years‚ and the reactivation
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Public Health at Washington University in St. Louis. While Rife was acquiring the technical skills and instruments he needed to isolate the cancer virus‚ Dr. Kendall was developing a protein culture medium for cultivation of the typhoid Bacillus. The typhoid Bacillus was usually large enough to be viewed through ordinary research microscopes of the time. However‚ when he used his Medium K‚ this bacterium changed into a form that was not visible or “non-filterable”. Then‚ it would morph back into a
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To scrutinize the color potency depth of the azo dye Remazol Black-B decolorizing activity by an effectual bacterial isolates‚ liquid medium was used. Based on the relative aptitude of the decolorization of diverse isolates‚ three most excellent performing bacterial isolates (1‚ 2 & 3) with more than 90% of decolorizing activity were preferred for more experiments (Data not shown). 3.1 Biodecolorization of Remazol Black-B by selected bacterial isolates Microbial decolorization of Remazol Black-B
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