Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
Premium Buffer solution Enzyme PH
Experiment 8: Hydrogen Phosphate Buffer Systems Purpose The purpose of the lab was to create a buffer solution and observe the capacity of the phosphate buffer system. Also‚ the experiment was meant to provide experience with the calculations and mathematics involved in creating the buffer solution. Finally‚ the lab was designed to provide an insight and appreciation for the necessity and complexity of buffers in our physiology and in the environment. Procedure No deviations were made from
Premium PH Acid dissociation constant Buffer solution
Objective: The objective of this experiment is to use acid-base extraction techniques to separate a mixture of organic compounds based on acidity and/or basicity. After the three compounds are separated we will recover them into their salt forms and then purify them by recrystallization and identify them by their melting points. Procedure: Extraction of Carboxylic Acid A pre-weighed (0.315g) mixture of Carboxylic acid‚ a phenol‚ and neutral substance was placed into a reaction tube (tube 1)
Premium Carboxylic acid Alcohol Acid
An Experiment to find the Concentration of a Sample of Acid Rain Plan Aim When a metal is extracted from its ore often sulphur dioxide is produced. When sulphur dioxide is dissolved in water it forms a strong acid called sulphuric acid. Sulphuric acid is often sold as a useful by-product‚ but also gets into the water cycle and forms acid rain. In this experiment‚ I have been given a sample acid rain of concentration between 0.05 mol dm-3 and 0.15 mol dm-3 and my aim is to find out exactly what
Premium PH Acid Sulfuric acid
ANSWERS TO QUESTIONS: 1. a. in distilled water: Cu(OH)2(s) (( Cu2+(aq) + 2OH-(aq) Ksp Cu(OH)2 Keq = Ksp Cu(OH)2 = 2.2 x 10-20 b. + 6.0M H2C2O4 Cu(OH)2(s) (( Cu2+(aq) + 2OH-(aq) Ksp Cu(OH)2 H2C2O4 (( H+ + HC2O4- Ka1 H2C2O4 HC2O4- (( H+ + C2O42- Ka2 H2C2O4 Cu2+ + C2O42- (( CuC2O4 1/Ksp CuC2O4 2(OH- + H+ (( H2O) 1/K2w Net Keq = (Ksp Cu(OH)2 x Ka1 x Ka2 )/Ksp CuC2O4
Premium Solubility Hydrochloric acid Chlorine
Abstract The bromination of trans-cinnamic acid was completed to determine dibromide’s stereochemical structure and its mechanism. After the addition of bromine to trans-cinnamic acid‚ the product was identified by its melting point and infrared spectrum resulting in erythro-2‚3-Dibromo-3-phenylpropanoic acid after comparing similar properties. Introduction In this lab‚ the bromination of trans-cinnamic acid was completed to determine dibromide’s stereochemical structure‚ and from there determine
Premium Solvent Acetic acid Ethanol
Abstract A buffer is a solution that resists changes in pH when H+‚ OH-‚ or H20 is added. By using standard lab equipment‚ a lab pro diagnostic tool‚ and acidic and basic solutions‚ the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid. Introduction The
Premium Acid dissociation constant Sodium hydroxide PH
Experiment 20: pH Titration: Phosphoric Acid in Cola Drinks Post-lab Assignment or Report The post-lab report for this experiment is due at the beginning of the following lab period. Student notes for the lab will be available on the lab T-Square site. Learning Objectives Students will be able to... • Use a known mass of solid acid to determine an unknown concentration of a basic solution (this process is called “standardization”). • Execute a titration using good‚ reliable technique.
Premium Acid PH Titration
Identifying Pb+2: 1) Took 2mL of my unknown solution and added 2 drops of HCl and got white ppt which correlates with the positive control. 2) Centrifuged the ppt and soln‚ then discarded soln and washed ppt and centrifuged again. 3) I added 2mL of H2O‚ put the test tube in the hot water bath and stirred for 3 minute with a stirring rod. 4) I then decanted the solution into another test tube. 5) I added one drop of 6M acetic acid and 3 drops of 1M K2CrO4‚ which formed a positive confirmation where
Premium Acid dissociation constant Base White
Name ___________________________________________Date experiment performed ________________________CRN of lab section: _______________________________ | Grade: ______________Days late: ____________Instructor Initials: _____ | General Grading Items | 25 Points | Attendance at Pre-lab Meeting | /10 | Copies of lab pages attached; labeled with name and date‚ complete information‚ readable‚ data recorded matches results given in report | /5 | Waste was properly disposed of and lab area was
Free PH Acid dissociation constant Acetic acid