IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Sophie Murdock Osmosis Lab Osmosis in Plant Cells: Solution Initial Volume (mL) Final Volume (mL) Percent Change Distilled Water 0.8 1.0 125% 0.4% NaCl 0.5 0.8 160% 0.9% NaCl 0.6 0.8 133% 5% NaCl 0.6 0.5 83% 10% NaCl 0.6 0.5 83% 8. The cells in the solutions of distilled water‚ 0.4% NaCl‚ and 0.9% NaCl would all appear to be inflated and could burst. This is due to the fact that the solution is hypotonic to the potato‚ thus water is entering the cell. Meanwhile‚ the cells in the solutions
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Retrieved October 29‚ 2013‚ from http://prezi.com/wii0fgw48euc/potatoe-enzyme-lab/ What enzyme is present in liver and potatoes. (n.d.). Retrieved October 29‚ 2013‚ from http://wiki.answers.com/Q/What_enzyme_is_present_in_liver_and_potatoes#slide2 What Is the Optimum PH for Catalase - Ask.com. (n.d.). Retrieved October 29‚ 2013‚ from http://www.ask.com/question/what-is-the-optimum-ph-for-catalase What is the optimum PH for potato catalase and why? - Yahoo! Answers. (n.d.). Retrieved October 29‚ 2013
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concentration. Hypotonic solution is the solution with the lower salt concentration. My hypothesis is that the potato core will increase in size. When the solution is hypertonic‚ the potato will decrease in size making the potato hypotonic. Osmosis is really interesting‚ that is why I chose to write this report about it. Materials and Methods We first cut out six cores out of a potato. We trimmed them all to the same length using a razor. After we had cut them to the same size we weighed them using
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Name: Angel Simon Pre–Lab Question Ocular lens 1. Label the following microscope using the components described within the Introduction. Experiment 1: Virtual Magnification Exercise Post-Lab Questions 1. At what magnification do you first notice the ragweed pollen? Answer: 1000 2. Which is bigger‚ a rhinovirus or E. Coli? Answer: E. Coli 3. Based on the magnification‚ how many of the E. Coli can fit into the same space as the
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How to Write a Lab Report Lab reports are formal papers that describe and analyze lab experiments. They follow a very specific format. If you study this paper carefully and follow its directions‚ you will find that these papers are not at all difficult and can have a positive impact on your Biology grade. Paper formatting: The paper should be typed and double spaced. Use a standard 12 point font (do not use cute fonts or colored paper!). Except for the title and abstract – each section
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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Labset Three Worksheet 1. What is a carbohydrate profile? Why are they used as a diagnostic or identifying tool? (2) Carbohydrate profiles are specific information on the type and amount of carbohydrate that a product contains. It is used to identify and differentiate two closely related species. 2. What are the carbohydrate profiles of the organisms you tested? (2) The yeast carbohydrate profile came out with glucose and fructose positive and Mannitol negative. The staph epidermidis
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Lab Topic: Movement of Materials Across the Cell Membrane—Potato Tuber Cells Introduction: Osmosis is a form of diffusion in which water diffuses through a selectively permeable membrane from a region of high concentration to a region of lower concentration. This form of diffusion takes place when the molecules in high concentration are too large to move through the membrane. Three factors that determine cell membrane permeability are shape‚ size‚ and polarity. For this experiment‚ it must
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Lab 10 Name: Complete the following then save with your name and attach to the dropbox for lab 10. Data Tables and Post-Lab Assessment Experiment 1 Post-Lab Questions 1. Label each of the arrows in the following slide image: A. Chromosomes B. Nucleus C. Cytoplasm D. Cell wall 2. What is the difference between the rough and smooth endoplasmic reticulum? 3. Would an animal cell be able to survive without mitochondria? Why or why not? 4. What
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