Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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bacterium Escherichia coli is being subjected to various environmental factors that affect the rate of growth. These factors scrutinized were the different types of nutrients‚ the intensity of aeration‚ or the temperature at which it was stored. The purpose of this lab is to determine which factor affects the Escherichia coli the greatest. It is known that these abiotic factors affect the rate of growth the greatest if they remain at the correct conditions for living. Escherichia coli and other
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Heat resistance of endospores of Bacillus pumilus Abstract Spore suspension of Bacillus pumilus was inoculated into universal bottles containing sterile distilled water in water baths at temperatures of 85°C‚ 90°C and 95°C. At specific time intervals‚ a sample was removed and spread on nutrient agar plates. The number of colonies formed was used to determine the D-value and z-value. The D-value for 85°C is 64.1 minutes‚ 25.7 minutes for 90°C and 8.2 minutes for 95°C while the z-value is 11
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University of Phoenix Material Major Anatomical Features of Insects Worksheet Part 1 Review the diagram and label the insect’s major anatomical features. Antenna Compound eye Head Thorax Leg Abdomen Part 2 In the following table‚ list the major anatomical features used for keying insects. Explain the morphology—structure and
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3. List 4 abdominal organs that you observed. Describe their position relative to each other using correct anatomical terms. (Rachel Mindel) The pancreas is located in the upper abdomen‚ is posterior to the stomach and some parts are in between both the stomach and the spine. The right side of the organ which is called the head‚ is nestled in the curve of the duodenum (juncture where the stomach meets the first part of the small intestine). The tapered left side of the organ is called the body
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CHAPTER 1 INTRODUCTION 1.1 PRELIMINERIES Due to highly development of chemical and biochemical industries‚ acetic acid has largely produced to fulfil the needs of world demand in production of daily used products and for pharmaceutical needs. Therefore‚ this study is carried out to introduce the production of acetic acid using biological pathways which includes the used of microorganisms. In this chapter will briefly explained on the knowledge about acetic acid and the microorganism
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2LIVER Anatomical overview What is liver? The whole liver together weighs 3-4 pounds. It is from a light pink to a dark red color. The liver is located right below the heart. The liver is a very big organ. The liver and gallbladder are responsible for a number of functions. One of the main functions is to help in digestion. The largest solid organ in the body‚ situated in the upper part of the abdomen on the right side. The liver has a multitude of important and complex functions
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Analyze the anatomical structure of ten different organelles in the cell and their respective functions. Ribosomes Protein formation Found on the surface of rough endoplasmic reticulum Two types 70S and 80S Centrioles Cylindrical in structure formed by microtubules. Participate in cellular division Chloroplasts It’s a type of plastids found in plant cells Contain green chlorophyll which helps in photosynthesis. Endoplasmic reticulum Found fused to nuclear membrane Serves as cell’s transport system
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Hand Soap Effectiveness on Escherichia Coli Abstract: Bacteria are an essential part of many different living organisms and ecosystems across the globe. Without bacteria there would have never had been life in the first place. But some bacteria have evolved to cause to humans‚ resulting in the need to eliminate them through such methods as soap. Hand soap is a crucial part of human health‚ especially in such places as schools and hospitals. In this experiment I wanted to determine the effectiveness
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animals being utilized in animal by-products and thereby to reveal when these products contained ingredients originating from animals that had died of anthrax. The thermostable antigens involved are common to other Bacillus species so the test depends on the fact that the only Bacillus likely to have proliferated within and throughout an animal depositing extensive precipitating antigens in the tissues is B. anthracis. Procedure: 1. Chop or slice the specimen into fine pieces or strips. 2
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