Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually iodine has a orange-yellow
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topic can understand the nature of the experiment. A good introduction is clear and concise. Carbohydrates and proteins should be explained in terms of their structure and major functions in cells. Your intro should end with a brief overview of the amylase experiment‚ including your hypothesis and predictions about what expect will happen in each treatment group of the experimentThe introduction is a statement of the subject and objectives of the experiment and presents your hypothesis. Relevant background
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enzyme amylase. The amylase is used to break down the polysaccharide starch. Amylase is a digestive enzyme classified as a saccharidase (an enzyme that cleaves polysaccharides). It is mainly a constituent of pancreatic juice and saliva‚ needed for the breakdown of long-chain carbohydrates (such as starch) into smaller units. Amylase is also synthesized in the fruit of many plants during ripening‚ causing them to become sweeter‚ and also during the germination of cereal grains. Grain amylase is key
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Amylase is found in the saliva of humans and is responsible for the first step in breaking down starch. Starch is a polysaccharide composed of a large number glucose monomers joined together. Amylase breaks down starch by separating the glucose molecules into maltose‚ which is a two glucose-unit compound. (Morgan‚ 2008) During this experiment we investigated the influence of pH on the activity of the enzyme Amylase. We are able to use the fact that when I2KI
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Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that‚ through its own structure including hydrogen bonds‚ acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process‚ without which cells could hardly practice any physiological functions within human
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enzyme‚ Amylase which is used to break down carbohydrates will work the best when heated at 40°C. Also‚ as the temperature increases the reaction rate of amylase increases too. However‚ the reaction rate of amylase will start decreasing when the temperature reaches the enzyme’s optimal temperature. Many enzymes are specific for a certain substrate. For example‚ lipase is a specific enzyme for fat substrates and protease‚ a specific enzyme for protein substrates. In the same way amylase is a specific
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INTRODUCTION Isolation of Aspergillus niger from garden soil for the production of amylase enzyme and effect of various parameters on enzyme‚ production is the main aim of this study. Enzymes are protein catalysts synthesized by living systems and are important in synthetic as well as degradative process. Amylases are starch degradative enzymes that catalyze the hydrolysis of internal a-1‚ 4-0-glycosidic bonds in polysaccharides such as starch in to simple sugar (glucose and maltose). It is produced
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Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity increases
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Exercise 8: Chemical and Physical Processes of Digestion: Activity 2: Exploring Amylase Substrate Specificity Lab Report Pre-lab Quiz Results You scored 100% by answering 5 out of 5 questions correctly. 1. Which of the following is true of enzymes and substrates? You correctly answered: c. Enzymes are specific about the substrates they can act upon. 2. Which of the following is/are reducing sugars? You correctly answered: e. both glucose and maltose 3. Cellulose and starch are both You correctly
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Title: Investigate the breakdown of starch at different temperature AIM This experiment has been done to investigate the action of the enzyme amylase on the breakdown of starch. MATERIALS These were the materials used: four starch/ agar plates‚ a marker pen‚ 1mm graph paper ruler‚ 8mm cork borer‚ forceps and template for cutting holes‚ 1% Amylase‚ water‚ incubator set at 5‚ 20‚ 40 and 60 degree Celsius. METHOD This was the experimental procedure carried out: the materials above were collected
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