LABORATORY 3 The Effect of Temperature on the Rate of PNPP Hydrolysis Partners: Shelby Cruickshanks Alexis Williamson Introduction Most of the chemical reactions‚ which occur throughout our bodies‚ would proceed at a much slower rate of reaction without the presence of an enzyme. Cells can not wait for centuries for molecules to break down‚ if they waited for that‚ there would be no way for the organism to obtain energy or in turn survive. This is where biological catalyst comes into
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Focused Question: How can one determine the presence of glucose‚ starch‚ lipid‚ and protein in various samples? Pre-lab Questions: • Glucose is a monosaccharide. What does this mean? A monosaccaride is a carbohydrate molecule with three to seven carbon atoms with the corresponding number of hydrogen and oxygen atoms. • Proteins are made of amino acids. What atom is present in an amino acid that is not present in a sugar molecule? One nitrogen atom is present per molecule in an amino acid
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Corrugating Starch Adhesives Manual Corn Products International‚ Inc. Quality Policy on Products‚ Services and Customer Relations It is the policy of Corn Products International‚ Inc.‚ to provide products that not only meet applicable government quality and safety standards‚ but also seek to meet evolving customer expectations for quality‚ value and service; and to assure the accuracy and propriety of all labeling‚ advertising‚ and other representations we make concerning our products and services
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This study focuses on the effect that temperature has on the enzyme amylase. Enzymes are composed of proteins and acts as a catalyst to speed up the rate of a specific chemical reaction by lowering the activation energy. Reactions do not need enzymes to occur‚ but the human body and other living organisms depend on the use of enzymes in order for biochemical reactions to happen in milliseconds. Each enzyme contains a distinct three dimensional structure which is related to its particular function
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temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction temperature of amylase solution
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LAB 10: NAME: DaeNia La Rodé DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of substrate concentration on the enzyme amylase INTRODUCTION: Enzymes are perhaps one of the most important proteins of the human body. Enzymes such as amylase‚ an enzyme that breaks down carbohydrates‚ work by means of surface catalysis. In other words‚ the surface of the enzyme enables other molecules to react in a manner they would not be able to without
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Introduction Starch is composed of α-amylose and amylopectin. The structure of α-amylose consists of long polymer chains of glucose units connected by an α (1-4) linkage. Amylopectin consists mainly of α (1-4) linked glucose residues but is a branched molecule with α (1-6) branch points every 24 to 30 glucose residues on average. As a result of the bond angles in the α (1-4) linkage‚ amylose actually forms a spiral much like a coiled spring. Amylose is responsible for the formation of a deep blue
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reaction. Each enzyme binds closely to the substrate; this greatly increases the reaction rate of the bounded substrate. Amylase enzyme‚ just like any other enzyme‚ has an optimum PH and temperature range in which it is most active‚ and in which the substrate binds most easily. The purpose of this experiment was to determine (1) the reaction rate of an amylase enzyme in starch and (2) the environmental factors that can affect the enzymatic activity. The hypothesis‚ in relation to the enzymatic
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Test for Carbohydrates Result Starch suspension When the yellowish-brown iodine was added into the starch suspension‚ the starch suspension was changed into dark blue. Potato slide Discussion Starch suspension When the yellowish-brown iodine was added into the starch suspension‚ the starch suspension was changed into dark blue. This is due to the yellowish-brown iodine changed into dark blue when there is a presence of starch. This shows a positive test for
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the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch and a buffer were combined at a
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