The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely high or
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correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to catalyze substrates. More enzymes means more reactions can take place at a time‚ thus a faster rate of reaction. Overall‚ based on the results of table 2.0 and graph 2.0‚ it is prevalent that there is a positive correlation between the concentration of enzymes and the rate of
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for the effects of temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity
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Lab Ex#8: "Enzymes: Catalysts of Life" INTRODUCTION Enzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity‚ we would not possess the catalyst activity necessary for energy to produce movement. Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables‚ such as temperature
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released
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Introduction Enzymes are key players in metabolism. A metabolism is the organic processes in a cell or an organism that are necessary for life. An enzyme affects the rate at which a reaction occurs when the activation energy is lowered. In this reaction the reactant is called the substrate which is that combine with enzymes molecules to form a temporary enzyme substrate complex. During this products are formed and the enzyme molecules released is unchanged. For the substrate complex to form the
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