are called pyrogens‚ which can come from inside the body or outside the body. 3. Salivary amylase and pancreatic amylase are released by two accessory organs of the digestive system. In what structures do the enzymes actually chemically digest food? * 4. Based on the information you have learned about the digestive system‚ describe the optimal pH for the action of pancreatic amylase. Do classroom experimental results seem to support this finding? Why might there be
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enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes environment will affect its activity rate. Discussion: In order to identify
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4A.4.2.2 Process Optimization: The process was optimized for two variables which can make difference in the particle size and further in the stability of emulsion.The process parameters varied were time of stirring and speed of stirring .The results are depicted in Table 4A.11 Process variables: Time of stirring: The batches were stirred for different time periods of 30‚ 45 and 60 minutes‚ keeping using magnetic stirrer. Speed of stirring: The speed of stirring was maintained at400‚ 600 and
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Abstract Over a two week period of time in the laboratory‚ we experimented and tested the reaction rate of a peroxidase enzyme and the factors that affected it‚ both positively and negatively. The purpose of these experiments was to probe and manipulate the activity of the enzyme peroxidase by varying temperature‚ pH‚ the amount of enzyme compared to the substrate and the effect of hydroxylamine. Peroxidase activity is expressed when the potato extract is subjected to stresses such as low temperature
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Jeopardy questions (2nd half) Old stuff 1. Draw and correctly label a graph to illustrate the principle of maintenance. 2. Who would have the higher blood lactate (all other things being equal): a. John who has more 2-3 DPG or b. Jay who doesn’t? Why? (A logical explanation must be provided for any marks) 2. What does M type LDH do? Nervous system 1. What part of the neuron is damaged with multiple sclerosis? 2. The resting membrane potential in neurons is generally in what range?
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The main focus of this experiment was to find the relationship between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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Natural enzymes are proteins that catalyze biological reactions by lowering the activation energy of the reaction without being altered during the process. The enzyme used in this experiment was the β-galactosidase purified from E. coli. This enzyme hydrolyzes lactose and turns it into galactose and glucose. Since it is difficult to assay the activity of β-galactosidase‚ we will be using the artificial substrate‚ o-nitrophenyl-β-galactoside (ONPG) instead of lactose. ONPG is an analog of lactose
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Enzymatic activity of Human saliva (Salivary amylase) against Temperature Proponent: Ian Angelo P. Dela Cruz BS-Biology 1-3 Prof. McJervis S. Villaruel Professor – BIOL2015(Lab) ABSTRACT This report entitled “Enzymatic activity of Human saliva (Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar
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temperature on amylase activity Design principle Background: Amylase activity products? (show the equation) Which factors will affect enzyme activity? How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products) Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at different desired temperature ranging from 0oC to100oC). Dependent variable: rate of amylase activity
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