Reaction Prof Declan Naughton PR MB 1074 Office Hours Friday 1‐3 pm (I) Introduction Alkaline phosphatase catalyses the hydrolysis of p‐nitrophenyl phosphate (a synthetic substrate) at an optimum pH of 10.0 with the liberation of p‐ nitrophenol. http://www.gbiosciences.com/ResearchProducts/PhosphataseAssay‐desc.aspx Aim: To determine the activation energy of the enzyme alkaline phosphatase (II) Method a) Preparation of a Calibration Curve b) Determination of Activation Energy of the Reaction
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diagnosis of AIP. In regards to lab values‚ the patient’s elevated IgG4 is not as diagnostic as one may expect as up to 10 percent of those with pancreatic cancer have IgG4 levels up to 280 mg/dL. Nevertheless‚ the disproportionate rise in alkaline phosphatase compared to ALT/AST seen here is fairly typical of
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Genomic Library A genomic library is a collection of recombinant vectors that represent the entire genome of an organism. Below is a brief outline of how to create the genomic library: 1. We isolated Vibrio fischeri DNA to pGEM genomic DNA using a phenol chloroform purification method. 2. Vibrio fischeri DNA and pGEM DNA was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared
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2% to 6% of the band neutrophils. They have a nucleus with a horseshoe or sausage shape they do not have a nucleus separated into lobes connected by a filament. The nuclear chromatin is clumped they stain various shades of pink and contain alkaline phosphatase. In normal peripheral blood of older adults they have 50% to 70% of segs. The nucleus is separated into two to five usually three lobes connected by a thin filament. Chromatin is clumped and stains purplish-red. Segmentation of the nucleus
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Factors||||| D-Dimer||||| Drug Therapeutic Levels:||||| Digoxin||||| Theophylline||||| Dilantin||||| Peak and Trough||||| Gentamicin||||| Vancomycin||||| Other||||| Arterial Blood Gases (ABGs):||||RA| ||||Supplemental L/min| pH (alkaline or acidity)||||| Partial pressure of arterial carbon dioxide (PaCO2 or PCO2)||||| Partial pressure of arterial oxygen (PaO2 orP02)||||| Bicarbonate Level (HCO3)||||| Saturation of arterial oxygen (SPO2)||||| Base Excess||||| Cultures:|||||
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of misfolded proteins‚ gram-negative bacteria use two major envelope stress responses (Raivio et al.‚ 2013). One of the envelope stress responses is the Cpx envelope stress response and it is activated by a diverse set of inducing signals‚ such as alkaline pH‚ chloride ions‚ copper‚ mutations that affect the folding of proteins in the periplasm‚ the overexpression of misfolded and aggregated periplasmic proteins at the inner membrane‚ and attachment to abiotic surfaces (Raivio et al.‚ 2013). These
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Developmental Alterations of Teeth II Amelogenesis Imperfecta pp: 99-106 All pictures are intellectual property of the Division of Oral and Maxillofacial Pathology or its Faculty. Duplication or any unauthorized use is prohibited. General Information • Classification can be impractical for clinicians • Problems arise in one or more of the three stages of enamel formation – Elaboration of enamel matrix; hypoplastic – Mineralization; hypocalcified – Maturation; hypomaturation General
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Background: Lipid and protein composition varies in tissue types. Results: When comparing liver‚ muscle‚ and adipose tissue‚ adipose tissue had the highest percent lipid and liver tissue had the highest percent protein. Conclusion: Based on analysis of the gels‚ the liver appeared to have the most variety in proteins when comparing liver‚ muscle‚ and adipose tissue. Significance: In order to determine the composition of a tissue‚ specific macromolecules can be extracted‚ quantified‚ and analyzed
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All glassware that was being used had to be cleaned so that other old substances did not interfere with the outcome of the results. This experiment included an unknown phosphatase buffer solution and enzymes A and B. The experiment had two sections. In Part A‚ a colorimetric assay was conducted by using a spectrophotometer. The substance was a clear‚ colorless liquid that turned yellow. This reaction occurred because p-nitrophenyl
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epigastrium‚ and right upper quadrant. No other mass‚ hepatosplenomegaly‚ or hernias noted. Pelvic and rectal‚ unremarkable. DIAGNOSTIC DATA: White blood cell count 10.1 with 79% segs. PTT is normal. SMA shows elevated bilirubin at 1.54 with alkaline phosphatase at 442. Sonography reveals multiple gallstones with a stone in the common bile duct. These were reviewed by Dr. Singh‚ radiologist. Chest x-ray was unremarkable. IMPRESSION: Cholelithiasis‚ choledocholithiasis‚ and early obstructed jaundice
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