Lab Report: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs‚ and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic‚ it must be produced by
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H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of O2 production. 3) Discard the used slide container. Results: Organisms Bubbles formation Catalase Patient G Bubbles Positive Micrococcus luteus Bubbles/O2 formed Positive Enterococcus faecalis No bubbles/No O2 Negative Observations: When H2O2
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of biopolymers often called PHAs or PHBs. The continual use of petroleum to make polymers for plastic will eventually run out and hence Biopol was introduced as a polymer replacement for petroleum. It is made from an organic substance known as Alcaligenes eutrophus which is a microorganism. Biopol is stable when stored in air and even humid conditions. When the polymer is exposed to microorganisms found in soil‚ sewage‚ river bottoms or other similar environments‚ the decomposition into carbon
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organisms to hydrolyze urea via the intracellular enzyme urease. Casein hydrolysis tests for organisms capable of hydrolyzing casein via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent
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INTRODUCTION: In this laboratory exercise explore the differences of microorganism and continue our use of specialized media and use some biochemical testing.This report discloses the basic laboratory instruments will be used in each of our practices .It is of great importance to recognize and identify the different instruments and laboratory tools‚ because in this way will we be able to use them properly and also to call them by name and know why. Listen Read phonetically Dictionary - View
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Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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Streak the plates for the following organisms: Enterobacter aerogenes‚ Salmonella enterica‚ and Staphylococcus aureus‚ and sterilize the loop after streaking each quadrant. 2. Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Streak the plate. 3. Repeat step 2 for Streptococcus pyogenes 4. Transfer an A disc with flamed forceps to the cross of first and second quadrant of the
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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Introduction The main purpose of root canal treatment is the elimination of microorganism from root canal system (1‚2). Microorganisms and their byproducts are regarded as the primary etiological factors of periapical diseases and pulpal necrosis (3‚4‚5). Microbial reduction obtained by mechanical instrumentation‚ irrigation‚ using intracanal medicaments and adequate filling of root canal (6). Failure of the root canal treatment is the result of persisting microorgansims in the root canal system
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