Gel Electrophoresis Lab SBI4U1 May 13th‚ 2013 Gel Electrophoresis Lab Purpose: The purpose of this lab is to learn how restriction enzymes cut DNA molecules at specific sequences‚ thus producing DNA fragments of various lengths. Students learn how fragments form unique patterns‚ which help to distinguish the base for DNA identification. This lab answers the question “whose DNA was left behind?”. Materials: * Transfer pipets * Agarose Gel * Dyed DNA samples *
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Procedure: Day 1: Buffer preparation First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.5 M Tris buffer at pH 6.8 3.033 g of Tris was weighed and placed in 400 mL beaker. Then‚ 25 mL of distilled water was added into the beaker that contained Tris. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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Stephen White Biology Lab 11.1 Observations of the spicules of a sponge Supplies Microscope Prepared slide: Sponge Lab notebook Colored pencils Natural Sponges Hypothesis We will learn more in depth about sponges and the complexity of this animal Procedure Set up microscop as instructed in previous expiraments. Place the prepared slide under the microscope. Obeserve under low power and draw what you see in your notebook. This slide shows you the spicules‚ wich make
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franciscana are known to live in high salinity lakes that are often basic (Biology 108 Lab Manual 2015). Furthermore‚ A. franciscana feed on photosynthetic phytoplankton which inhabit areas of light availability but are also more susceptible to predation in highly-lit areas (Biology 108 Lab Manual 2015). Also‚ A. franciscana can withstand a broad range of temperature except extreme values may affect survival (Biology 108 Lab Manual 2015). In this experiment‚ the habitat
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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Destiny Thomas Biology October 19th 2012 Period: 3rd Destiny Thomas October 19th 2012 Biology Period: 3rd Introduction In our experiment we used only the red colored life savers. They all had about the same mass. We used 9 life savers. In our experiment we had to dissolve our life savers. The concept of dissolving is to place the
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Function 16 October 2014 Gel Filtration and Electrophoresis Objective The essential goal of the experiment was to separate proteins in a solution based on size in different fractions. The relative protein content for each one fraction was found through the utilization of an amido black-based protein assay. Later in the trial polyacrylamide gel electrophoresis was utilized to separate BSA from hemoglobin. Methods I. Gel Filtration and Protein Assay: 1. A slurry of Bio-Gel P-100 beads in water was
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quantities present in the urine. APPARATUS : 1) Test tubes 2) Boiling water bath 3) Microscope 4) Slides and cover slips 5) Pasteur pipettes MATERIALS: 1) pH test strips 2) Benedict’s reagent PROCEDURES: Collect the urine samples in a container. Make sure to not spill anywhere in the lab. Complete the tests and all results/ observation are in a proper table. (A) Observation of urine 1) The urine is sniffed and the description of the urine is described. Terminologies like slightly
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Shalacia Gilmore Fall 2012 BIO 1107 Natural Selection Lab INTRODUCTION In the 1850s‚ two scientists by the name of Charles Darwin and Alfred Russell Wallace composed the theory of evolution by natural selection. (1) Darwin characterized several claims needed for natural selection to happen‚ including heritable variation within the population‚ and the presence of more individuals than the environment can support. They also discovered that certain environments favored certain traits. These
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EXPERIMENT REPORT (PREPARATION OF CdS) Experiment Purposes 1. To master the proper operations as well as the suitable situations of atmospheric pressure filtration‚ vacuum filtration‚ centrifugal separation & precipitation washing 2. To understand the relation between precipitation state % precipitating conditions 3. To learn the way to examine whether reactants are fully precipitated 4. To learn the principals of powder material synthesizing through room temperature solid phase method
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