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    Lab: Sampling Bacteria

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    variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar upon feeding bacteria to it. 2. Why shoudn’t a student swab his or her mouth or cough onto an agar plate to initiate a culture? Even though most bacteria in the human body is harmless‚ if one was knowingly or unknowingly ill then harmful bacteria

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    Unknown Lab Report

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    Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide

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    CHAPTER ONE 1.0 INTRODUCTION Natural rubber is produced by over 2000 plants species and its main constituent is poly (cis-I‚4-isoprene).a highly unsaturated hydrocarbon. Since 1914 there have been efforts to investigate microbial rubber degradation: However‚ only recently have the first proteins involved in this process have been identified and characterized and have the corresponding genes cloned. Analysis of the degradation product of natural rubber and synthetic rubbers isolated from various

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    IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus

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    Pglo Transformation

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    pGlo plasmids. It was hypothesized that if bacteria that were transformed with +pGlo plasmids are given the gene for GFP‚ then transformed cell colonies will be located on the LB/amp/ara and LB/amp agar plates. Cells that have been transformed with +pGlo plasmids have the ability to grow in ampicillin plates‚ and the arabinose sugar allows the colonies to be visibly fluorescent under ultraviolet light. The GFP is able to resist ampicillin because of the Beta- Lactamase protein that is produced and secreted

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    result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis ‚ my organism produced gamma hemolysis on the blood agar because there was no hemolysis on the plate after 48 hours incubation period. After examining my unknown for hemolysis I set a series of five experiments: Bacitracin SX CAMP Enterococcosel 6.5% NaCl My unkown

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    were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect the results of microscopic observations as well as the differential tests results on various agars and broth cultures. Although all tests were not conclusive‚ the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens. INTRODUCTION The field of Microbiology is the

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    Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize

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    ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and

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    A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot

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