The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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ATCC 8007. The bacterium was activated in Man-Rogosa-Sharpe (MRS) agar medium (Difco‚ BD Diagnostic Systems‚ Maryland‚ USA) consisting of (g L1): peptone casein‚ 30; meat extract‚ 10; yeast extract‚ 6.0; sodium acetate‚ 5.0; ammonium citrate‚ 2.0; glucose‚ 0.2; magnesium sulfate‚ 0.2; manganese sulfate‚ 0.05; dipotassium phosphate‚ 2.0. The pH of the medium was adjusted to 6.5. The master cell bank culture was inoculated into agar plates and incubated for 48 h to produce the working cell culture. Stock
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MASINDE MULIRO UNIVERSITY OF SCIENCE AND TECHNOLOGY P.O. BOX 190-50100 KAKAMEGA FACULTY OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree
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Abstract Introduction An antimicrobial is a substance that kills or inhibits the growth of microorganisms such as bacteria‚ fungi‚ or protozoans (Antimicrobial). Antimicrobial drugs either kill microbes or prevent the growth of microbes. Disinfectants are antimicrobial substances used on non-living objects or outside the body. Ginger Figure 1 : Ginger (Studies Reveal Ginger Lowers Colon Cancer Risk) Ginger is commonly used around the world and has been employed in the treatment‚ cure‚ and
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bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab sens test. A known Gram positive coccus bacteria
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Effect of chalepensin on S. mutans growth Fifty microliters of a S. mutans suspension (1 x 103 bacteria/ml) in BHI medium (Remel‚ Lenexa‚ KS) were transferred to flat-bottomed 96-well plates (Corning Incorporated‚ Corning‚ NY) containing serial dilutions (1:2) of 50 μl of chalepensin‚ or 1 μg/ml tetracycline‚ chalepensin-free vehicle (vehicle was similarly processed as with chalepensin extraction‚ but without plant material)‚ and culture medium controls‚ and incubated for 6 h at 37°C. Next‚ MTT was
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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setting them above the cone of the flame to make the loop red‚ then left them to cool in the test tube tack for streaking. The media included a liquid (YED media that was made by 3g yeast extract‚ 6g anhydrous dextrose‚ 6g agar‚ and 300 mL tap water). The liquid media was on a hot plate‚ then an oven mitt was used to carefully transfer onto 4 petri dishes at a tilted angle‚ with the petri dish lids at an angle as well to reduce airborne contamination. After the end of each pour—no more than 2/3 of the
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Continental Drift‚ Plate Tectonics‚ The North American Plate The experts and scientists have theories on how the the world’s plates move and how earthquakes occur. These theories on how the world’s plate move is called continental drift and the theory on how earthquakes occur is called plate tectonics. They also have a lot of information on the North American Plate and in which direction it is moving and information like that. Now let me tell you more about each of these topics. Continental
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that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your hand‚ but not so much that the agar becomes solid again. Perform the following transfer as quickly as possible. You must work rapidly so that the liquid agar will not cool and solidify before the transfer is completed. 3. Hold both a test tube
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