identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol red sucrose‚ the SIM test‚ H2S by SIM‚ IMViC (indole‚ motility‚ voges-proskauer‚ and citrate)‚ Urease (urea broth)‚ PDase (Phenylalanine Deaminase)‚ Lysine Decarboxylase‚ and Ornithine Decarboxylase. Colonial morphology on EMB was used to
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Doctors use this to determine which antibiotics are needed to treat infections. b) The test is used to see how the antimicrobials impregnate on the disks are then placed on a plate which then are incubated to grow more bacteria it also allows the agar to diffuse over. Then a concentration gradient is created‚ if there is a zone of inhibition the the disks will show the growth being
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dichromate is an orange inorganic chemical reagent and methylene blue is a chemical compound commonly used for staining because of its color blue. These three substances have 158 g/mole‚ 294 g/mole and 374 g/mole‚ respectively. A jelly-like substance‚ agar-water gel in a petri dish was used as a medium for diffusion since water and air‚ as a medium‚ are not controllable
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on bacterial growth. By observing the effects of 5 different inhibitors including alcohol‚ bleach‚ soap solution and distilled water‚ it was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial culture on top. Using forceps‚ a filter disc was dipped into each inhibitor and then into a separate quadrant of the Petri dish. The lid of the Petri dish
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LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO.- 10800037 ROLL NO- RB1R07B02 B.TECH BIOTECH.(8th sem.)
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Microbes are everywhere. Objectives: The experiment performed in the Lab was isolation of microbes taken from us and the environment. We used Nutrient Agar which is a growth medium used to culture microorganisms or small plants and Sabourand Dextrose Agar plates used to cultivate moulds and yeasts. The objective of it was to demonstrate that microbes are everywhere. We expected to find a variety of bacteria‚ moulds and yeasts. We were introduced to aseptic techniques as they help ensure that
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a colony count. Sterile cotton swabs were used to sample the classroom telephone‚ light switch‚ cell phone‚ and a student’s tongue. After carefully swabbing each site‚ we inoculated separate Petri dishes and a test tube of nutrient broth. A blood agar plate was exposed to bacteria by coughing on it. The plates were then incubated at 37ºC for 48 hours. The light switch and telephone were contaminated with both bacteria and mold which produced turbid tubes of broth. However‚ the sampling of the
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generating diversity in life and aiding in evolution. Materials and Methods The materials used in the lab were two agar petri plates‚ a permanent marker‚ labeling tape‚ sterile scalpel‚ compound microscope‚ toothpick‚ microscope slide‚ water‚ cover slip‚ and the ascospore Sordaria cultures‚ which included wildtype (dark)‚ tan‚ and grey (mutant) fungi. First‚ collect two agar plates to make the cross between the three different colored ascospores. The Sordaria should be crossed in the following
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Petri dish- This is used to transfer the agar cubes as you should put the cubes in there as some of the chemicals can be dangerous Safety goggles- These are essential to keep your eyes protected from harmful chemicals White tile- To help to see the colour change Stop clock- To time how long it takes for the cube to change colour. Method 1-Stop the clock and quickly but carefully place one of the agar cubes into the specimen tube containing 3.7g/100cm3 of hydrochloric acid
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s Disease can be divided in two main groups infections and non-infections. Infectious diseases are diseases that can be spread or transmitted from one organism to another. Non – infectious diseases cannot‚ be spread from one person to another. Infectious Diseases Infectious diseases are generally caused by microbes known as pathogens. Pathogens invade the host and can cause changes in the body that stop parts‚ or all‚ of the body working correctly. Pathogens contain antigens that are made of
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