OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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Title: Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Title : Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Objective : To screen for thermophilic fungi as producer of fungal cellulase. Introduction One of the most important sources of carbon that is abundantly found on this planet is cellulose. While cellulase is the enzyme to degrade this carbon and it is a key enzyme in the bio refinery process of producing green chemicals
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Case Analysis China Dolls Issues : the company is about to lose two major customers due to “cheaper” price‚ namely China. Alternatives: 1. Move to China Closing down partially Malaysian and Chieng Mai operations 2. Expand to China Retain Malaysian operations Joint venture 3. Develop own label for Malaysian and ASEAN market. Costs : i. Set-up costs (investment) ii. Operation costs (labor cost) iii. Sunk costs iv. Opportunity costs v. Human ‘cost’ Explanations
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aumenta a cada ano que passa‚ ocasionando um crescimento de orkuts‚ blogs‚ e-mails e também‚ divulgação de vídeos. O que muitos não sabem é que esses textos‚ vídeos e imagens‚ dependendo do conteúdo‚ podem ser considerados um crime. Entre eles‚ os mais comuns na internet são: pornografia infantil‚ prática de estelionato‚ divulgações de conteúdos ilegais‚ violação de sigilo de correspondência eletrônica e‚ principalmente‚ acusações‚ e questões que envolvam a calúnia‚ injúria e difamação. O doutor
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well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow. This experiment allowed us to observe the process of bacterial transformation. I believe that only a small percentage of the cells will transform and the gfp plasmid will be most apparent in the agar plate containing both the plasmid and ampicillin.
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ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
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some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium‚ the bottom of the BHIA dish was labeled with the bacterium number‚ initials‚ and section; then divided into four quadrants. The second step‚ we used the septic technique to transfer a small
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Gram-negative bacteria and fungal strains by measuring zone of inhibition. The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5‚ 5.0‚ 7.0‚ 10µg/ml respectively. Ampicillin (antibacterial)‚ Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in
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inoculating loop and dry heat technique‚ isolation streak was performed and nutrient agar plates were to be incubated in a room temperature for the next 48 hours. Nutrient Agar plate was used for isolation streak technique in order to see two types of bacterium growing in a room temperature. After incubating for 48 hours Nutrient agar plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification‚ those
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procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. This was done by streaking the unknown onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed
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