A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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INTRODUCTION Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature‚ this plant has been described to be useful against skin diseases‚ liver troubles‚ tuberculous glands and its use in the treatment of rheumatism‚ hematemesis‚ pruritus‚ leucoderma‚ and diabetes.[1‚2] Besides‚ it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women
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(RESEARCH TREATISE) By SITUMBEKO LIWELEYA (s213459531) Submitted in fulfilment of the requirements for the degree of BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY At the At Nelson Mandela Metropolitan University Port Elizabeth‚ 2013. SUPERVISOR- PROFESSOR SMITH. N. DECLARATION I‚ the undersigned‚ hereby declare that the research work contained in this study is my own original work‚ and all the sources I have used or quoted have been indicated and acknowledged
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Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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Laguna. B. Maintenance of Microbial Cultures Bacterial isolated were subculture on slants of Nutrient Agar (NA). Incubation of the subcultured specimens for bacteria was done for 7-14 days in Isolation Room. C. Media Preparation for Assay Three tubes with nutrient agar were prepared. The cultures were inoculated onto their respective media by placing 0.2ml of each. The inoculated agar was then poured to individual sterile plates. D. Extraction from Leaves The extraction was done through
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• Eye protection • 250 mL beaker • Timing device • Scoopula • Ruler • Scalpel • Sodium hydroxide solution • 3 different sized cubes of phenolphthalein agar • Paper towels Purpose 1. Put on eye protection 2. With the scalpel cut the block of phenolphthalein agar in to a 1x1x1 cm cube (Cube A)‚ a 2x2x2 cm cube (Cube B) and a 3x3x3 cm cube (Cube C). 3. Pour enough sodium hydroxide solution in to the beaker to cover the
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since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame of the laboratory burner three times in rapid succession
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for specific organisms. For i.e. Mackonkey agar only grows on gran(-). If a sample is negative it grows and if it is positive it doesn’t grow. Last but not least Differential media relies on color change of the media itself. This color change highlights the bacteria if it has a specific trait. For i.e. If sugar ferments there will be a color change. III. Reagents: 1.Tryptone 2.Yeast extract 3.NaCl 4.KCl 5.MgCl2 6. MgSO4 7.Glucose/ Dextrose 8.H20 9.Agar( for solid) IV. Methods Grab an Erlenhymer
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Resumo texto do Sartori - Igualdade Ideologias Mundiais Maria Manuela Coelho Um ideal de Igualdade Para buscar a igualdade não podemos nos dar ao luxo de relaxar. Taney dizia‚ fazendo eco a Rousseau: “Enquanto a desigualdade é fácil‚ pois não requer mais do que se deixar levar pela correnteza‚ a igualdade é difícil pois significa nadar contra ela.”. Desigualdade X Igualdade A desigualdade pode ser atribuída a atos divinos‚ enquanto que a igualdade só pode resultar de atos humanos. Desigualdade
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the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH4OH‚ M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm)‚ resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl‚ M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed
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