Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
Premium Ultraviolet DNA Plasmid
Kayleigh Schmidt Purpose 2: Exercise 1 Part A Purpose: The purpose of this lab is to explore inoculation‚ incubation‚ isolation‚ inspection‚ and identification. Inoculation is the placement of a small sample or cells or the material with cells on a culture media that helps these cells grow. Incubation involves the optimal growth temperature in a controlled setting and is when the cells start to become visible. There are two types of inspection‚ macroscopic and microscopic‚ and it helps to identify
Premium Agar plate Bacteria Temperature
Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
Premium Bacteria Metabolism Enzyme
Lab Session 8: Selective and Differential Media‚ Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ ________ Objective: Analyze microbes from last week. Understand the use of antibiotics on microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol
Premium Agar plate Growth medium Bacteria
were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect the results of microscopic observations as well as the differential tests results on various agars and broth cultures. Although all tests were not conclusive‚ the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens. INTRODUCTION The field of Microbiology is the
Premium Microbiology Bacteria Staining
my thanks to the Principal of Western College of Commerce & Business Management‚ for extending his support. My deep sense of gratitude for providing Computer lab for completing 80 hrs. Thanks and appreciation to the people who supported for completing 40 hrs at the community level. I would also thank my Institution and my computer lab technician & attendant. I also extend my heartfelt thanks to my family and well wishers. Reason for joining Extension work Activity I have joined the department
Premium Adult education Lifelong learning Education
Overview A transistor is a semiconductor device that amplifies and switches electrical currents. They are the core component of any modern electronic devices‚ such as computer‚ telephones and other electronics. Nowadays most transistors are use to produce integrated circuits. There were a numerous inventions‚ or problems with the inventions that lead to the birth of the transistor. Radio signals‚ could be sent carrying information over a long distance away; the only problem was there was no device
Premium Transistor Integrated circuit Vacuum tube
MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
Premium Gram staining Staining Escherichia coli
Microbiology Lab Report II Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to
Premium Escherichia coli Microbiology Agar plate
disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance from the disk increases. If the compound is effective against bacteria at a certain concentration‚ no colonies will grow wherever the concentration in the agar is greater than or equal to that effective concentration. This region is called the
Premium Antiseptic Bacteria Microbiology