2 were Pseudomonas aeruginosa. One of each was used to confirm. Finally only MTCC cultures were used to prepare inoculum. Inoculums Preparation Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar‚ MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial
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Twentieth-century actor John Smith once said‚ “Sliced bread is only as good as the peanut butter and jelly on top of it.” John Smith like many people delights in the traditional American peanut butter and jelly (PB&J) sandwich. The combination of salty like peanut butter and a sweet taste of jelly brought great sensation in turn of the 20th century. The simplest in making the sandwich and how affordable it is‚ makes it an ideal household meal. PB&J sandwiches appear in mostly all American food places
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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Hoi Tin Tong Analysis Hoi Tin Tong is celebrated for its main product‚ Herbal Jelly namely Gui Ling Gow which is made of a kind of Chinese medicine formula. The marketing mix (4Ps) will be discussed in details in the following paragraph in order to have a general view of the company. Product Different varieties Products like Fresh Herbal Jelly‚ Pearl Turtle Jelly‚ Gui Ling Gow were found. Obviously‚ different ingredients are added to attract people from two aspects‚ the flavor and
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’Diamond’‚ or ‘Chloe’‚ and definitely not a ‘Daisy’; then it hit me‚ ‘Jelly’. As I laid on
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describe this phenomenon [2]‚ but is this just a neat trick‚ or can some species really live forever? This jelly is now known as the “immortal jelly”‚ and its infamy has grown with the years. But no one has published a report that this jelly can truly withstand the test of time; in fact‚ only one paper has been published suggesting some jelly relatives could live forever. Like many jelly species‚ members of the group Hydra have a polyp stage that reproduces asexually by budding off little clones
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others. Two examples of selective medium are Mannitol Salt agar and Phenylethyl Alcohol. 2. What is a differential medium? What makes the medium differential? Name 2 examples (3 pts.) Differential medium is distinguishing microorganisms from one another based on growth characteristics. A medium is differential when you are able to visibly see the differences in growth patterns of organisms. Differential media include blood agar and Eosin Methylene Blue. Steps Used in Identifying an Unknown
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Microbiology and Process Analysis laboratory 25/10/2013 Group 1 Microbiology laboratory Abstract The lab exercises were divided into three different analysis; microscopy‚ soil microbiology and bacterial growth. The main aim of laboratory work with Escherichia coli and soil sample was to introduce students to bacterial growth in pure culture and soil microbial flora. The experiment of bacterial growth in pure culture using optical density
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points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 4. Using sterile technique place disk in each of the solutions:
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Medical Microbiology II - Laboratory Report 1 Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral
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