small end bent to close it. * 1 Sharpie marking pen. * 1 glass test tube with a cap containing 2 ml of sterile nutrient broth and labeled "Broth". * 2 Petri dishes containing only nutrient agar and labeled "No Amp" on the bottom with date. * 2 Petri dishes containing nutrient agar and the antibiotic ampicillin. The dishes should be labeled "Amp" on the bottom with the date. * The laboratory instructions. Methods: Pre-Lab: * PREPARATION OF THE E. COLI STARTER
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This is a quantitative test and is considered to be the gold-standard method for biofilm detection (13). Organisms isolated from fresh agar plates were inoculated in 10 mL of trypticase soy broth with 1% glucose. Broths were incubated at 370C for 24 hours. The cultures were then diluted 1:100 with fresh medium. Individual wells of sterile 96 well-flat bottom polystyrene tissue culture
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Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test
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Digital image. Flickriver.com. Flickriver Viewer Software‚ 2007. Web. 3 Nov. 2013. An endospore stain of Bacillus cereus using malachite green. Digital image. Academic.missouriwestern.edu. N.p.‚ n.d. Web. 3 Nov. 2013. Hahn‚ Brittany. Phenyl Ethyl Alcohol agar. Digital image. Studyblue.com. STUDYBLUE INC.‚ 22 Apr. 2013. Web. 3 Nov. 2013. Reynolds‚ Jackie. Selective and differential media. Digital image. Www.microbelibrary.org). Kern Community College District‚ 12 Sept. 2011. Web. 3 Nov. 2013. Rose‚ Scott
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was in order to test the many effects of diffusion and osmosis amongst four experiments. One such experiment was testing the effects of molecular weight on diffusion in relation to the use of Agar. The methods performed included the use of two acids‚ HCl and acetic acid. Both acids were placed into an Agar-filled dish and‚ over increments of 15 minutes‚ data collection was taken based off the diffusion rate and the diameter length of both the HCl and the Acetic Acid. The resulting factor was the
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Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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was clearing off the DVR and catching up on old episodes of The Martha Stewart Show‚ I spotted an episode description that said “Jelly Mongers”. I’ve been in a canning mood this summer‚ so I figured this would be something informative for me to watch. Well‚ it turns out that it WAS very informative‚ but it also turned out to NOT be about canned jelly. Of course the “jelly” referred to was Jell-O basically. I know from my studies of historic culinaria (and endlessly watching British movies and TV shows)
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Pharmaceutical Microbiology CLASS CODE: MP202 SARA ASHOUR Signature ……………………………….. Date ……………………………….. Total Word Count 1492 I declare that‚ except where specifically indicated‚ all the work presented in this report is my own and I am the sole author of all parts. Toxic shock syndrome (TSS) is an uncommon but serious systemic infection which can occur in males and females of all ages. Symptoms include fever‚ hypotension
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quickly diffusion occurs is dependent on several factors‚ such as temperature‚ particle size‚ and the concentration difference on either side of the membrane. Testing the hypothesis relating to the effect of molecular weight to the diffusion rate‚ agar plate set-up was used to observe the diffusion of different substances. The methylene blue and potassium permanganate (KMnO4) was used and placed in the plate. Because the two substances have distinct colors‚ the measurement of the diffusion zone was
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Moon Cake Festival: A Mid-Autumn Festival (Chung Chiu)‚ the third major festival of the Chinese calendar‚ is celebrated on the 15th day of the eighth month. This festival corresponds to harvest festival s observed by Western cultures (in Hong Kong‚ it is held in conjunction with the annual Lantern Festival). Contrary to what most people believe‚ this festival probably has less to do with harvest festivities than with the philosophically minded chinese of old. The union of man’s spirit with nature
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