well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow. This experiment allowed us to observe the process of bacterial transformation. I believe that only a small percentage of the cells will transform and the gfp plasmid will be most apparent in the agar plate containing both the plasmid and ampicillin.
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inoculating loop and dry heat technique‚ isolation streak was performed and nutrient agar plates were to be incubated in a room temperature for the next 48 hours. Nutrient Agar plate was used for isolation streak technique in order to see two types of bacterium growing in a room temperature. After incubating for 48 hours Nutrient agar plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification‚ those
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Gram-negative bacteria and fungal strains by measuring zone of inhibition. The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5‚ 5.0‚ 7.0‚ 10µg/ml respectively. Ampicillin (antibacterial)‚ Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in
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skewer with petroleum jelly‚ then it should go through the balloon without popping. Hypothesis for the 2nd part of the Lab: If we cover the side of the balloon with cellophane tape‚ then it will not pop when pierced with the pin. Materials: • Balloons • Long wooden skewer • Petroleum jelly • A sharp pin • Cellophane tape • A pencil • Paper Pre-Lab Questions: 1. The balloon is being tested and analyzed 2. We are using sight and touch 3. Independent= petroleum jelly Dependent= the balloon
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What Sunscreen really works? Problem: Will sunscreen with an SPF of 4 be able to withstand the sunlight more effectively than SPF 45? Purpose: Due to the increasing amount of sun exposure on teens‚ we want to ensure that sunscreen can protect against the sun. An increase amount of exposure can damage the skin directly. The dangers of increased sun can lead to the 3 types of skin cancer (Basal‚ Melanoma‚ and Squamous) and these types can spread to other parts of the body. The most dangerous form
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aureus were maintained on Tryptic Soy agar (TSA)‚ following incubation at 37 oC for 24 h. Inocula were obtained from overnight fresh cultures adjusted to approximately log 108 CFU/ml‚ a turbidity equivalent to a 0.5 McFarland standard (Kroning et al.‚ 2016). The inocula were spread on the surface of Mueller-Hinton agar (MHA) plates. The disk diffusion test‚ recommended by the Clinical and Laboratory Standards Institute (CLSI‚
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WUS101/2 TERAS KEUSAHAWANAN SEM II 2012/2013 LAPORAN PROFIL USAHAWAN Prepared by: NORHAMIZAH BINTI IDROS 108965 930623-10-7864 KEJURUTERAAN ELEKTRONIK (EE) Prepared for: MOHD KAMIL ARIFFIN LECTURER WUS101 USM 25 APRIL 2013 Date: 20 APRIL 2013 Norhamizah Idros Kampus Kejuruteraan Universiti Sains Malaysia 14300 Nibong Tebal‚ Pulau Pinang. Mohd Shafie Ariffin Lecturer of WUS101 Universiti Sains Malaysia. Submission of WUS101
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. This was done by streaking the unknown onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed
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