Abstract Microorganisms are plentiful and widespread in the environment. In this lab‚ we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab
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count) of the total viable bacteria in the rice salad on a general non-selective agar using either the pour or the spread plate method. To confirm that the outbreak had been caused by any B. cereus present in the rice salad a selective media agar‚ such as mannitol egg yolk polymixin agar (MEYP/MYP)‚ should be used. Once B. cereus has been confirmed a further enumeration of the B. cereus should be performed on the MEYP/MYP agar selective media plate to show whether the amount of B. cereus present is within
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of different concentration of protease. In this experiment‚ a plate of milk agar is needed for comparing the result of each sample. Circular wells were dug on the milk agar plates by the use of cork borer‚ and extracts could be put into the wells by dropper. One must note that milk-agar is white in color due to casein. If protease is present‚ casein will be digested by the enzyme giving a clear zone in the milk-agar plate. The size of the clear zone can help us to determine whether fruits differ
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urease. Casein hydrolysis tests for organisms capable of hydrolyzing casein via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse
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Advances in Fish Microbiology and Pathology (FIS 508) Dr. Akinyemi‚ A. A. Aquaculture and Fisheries Management University of Agriculture‚ Abeokuta‚ NIGERIA. Microorganisms • Microorganisms is the existence of every minute living organisms or they are living features that can be seen with the aid of microscope‚ microscope‚ most of them are normally singlecelled while some may exist in multicellular forms. • These microorganism‚ though minute and microscopic‚ are a very powerful
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the Gram stain‚ and light microscope identify at least 2 Prokaryotes (bacteria) found in the water samples that are isolated on the MacConkey agar plates and the nutrient agar plate. Using the Identification Lab manual‚ identify at least 2 Eukaryotes (fungus) found in the soil sample that are isolated on the Potato dextrose agar plate and the nutrient agar plate. 3. In an agricultural context‚ research bacteria and fungus and their importance to Earth. 4. A high quality‚ 3+ resource
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early days of microbiology in the 19th century‚ culture on agar plates has been a central technique for the study of bacteria. This practical is designed to introduce students to the basic techniques required to manipulate bacteria. Students will gain experience with the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture
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Title Antimicrobial properties in different type of plants. Introduction A substance that kills or prevents the growth of microorganisms for example bacteria‚ fungi or protozoans is called an antimicrobial. This substance has 2 major roles which are to either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects outside the body. This substance included antibiotics‚ antifungals‚ antiprotozoals and
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is believed that higher temperatures will assist in the growth rate in the agar plates. Therefore it is believed that the agar plates placed in full light will produce more bacteria. Due to the type of light used for the full light part of the experiment there will be higher temperatures and therefore grow better than the no light and day light plates which are at lower temperatures. Equipment and Materials: 1 6 agar plates 2 E. coli and S. albus bacteria 3 Light 4 Day light 5 Cupboard
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alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable‚ 1 Pencil‚ marking‚ 11 Petri dish‚ 60 mm‚ 2 Candles (flame source)‚ 1 Thermometer-in-cardboard-tube‚6 Test Tube(6)‚ 16 x 125 mm in Bubble Bag‚ 1 Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated Small (5 mL)‚ 1 Baker’s Yeast Packet – Saccharomyces cerevisiae‚ 1 Agar‚ MRS - 18 mL in Glass Tube‚ 4 Agar‚ Nutrient - 18 mL in Glass Tube‚ 1 Broth
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