stain. * Observe the slides under microscope and record results. BIOCHEMICAL TESTS SUSCEPTIVITY TESTING Materials * MHA agar * Organism grown in Broth * Swabbing stick * Principle Procedure TSI (TRIPLE SUGAR IRON TEST) Materials * TSI agar * Straight wire loop * Cultured colony of organism * Purity plate Principle TSI agar detects fermentation of glucose 0.1%‚ lactose 1% and sucrose 1%‚ production of H2S‚ and gas formation. The medium contains ferric
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Yellow | ColourlessMedia: Pink | ColourlessMedia: Yellow | Pink-RedMedia: Pink | ColourlessMedia: Pink | E. coli grown in EMB agar: As we can see from the table above‚ the streak of E. coli on a plate with EMB agar showed a metallic green sheen where E. coli was present. EMB stain is selective for gram-negative bacteria. It is made using 6:1 Eosin and Methylene Blue. EMB agar is a differential media and inhibits the growth of gram-positive bacteria while also using a colour indicator to tell the difference
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bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to precipitate the DNA to see if the bacterium had the enzyme DNase. If the test could have been analyzed‚ Staphylococcus aureus would be positive for DNase and E. coli would have been negative
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and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special
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Bacteria will be grown in an agar broth containing .01%‚ .1%‚ .5% and 10% antibacterial soap. To fully understand the subject‚ information on everything necessary to conduct this experiment and comprehend the results was researched. Once the experiment is completed a conclusion will be able to be made as to whether or not bacteria can become resistant to antibacterial soaps The procedure for the experiment is as follows. Ecoli bacteria will be grown in agar broth with .01%‚ .1%‚ .5% and 10%
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TCBS agar is a selective as well as differential culture medium used for isolation of Vibrio spp. The growth of intestinal flora other than Vibrio spp. is prevented by the high pH (8.5-9.5) of TCBS. TCBS agar consist off yeast extract‚ bacteriological peptone‚ sodium thiosulphate‚ sodium citrate‚ ox bile‚ sucrose‚ sodium chloride‚ ferric citrate‚ Bromothymol blue/ thymol blue and agar. Sodium thiosulphate‚ sodium citrate and ox bile are the components
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Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
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of common household spices/herbs such as cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic‚ on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator)‚ garlic will have the strongest antimicrobial effect. This is because
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such as the mouth‚ blood vessels‚ heart and lungs and make up the outer layers of the skin. The bottom layer of the cells is attached to the basement membrane for support and connection. The main function is allowing materials to pass through via diffusion and osmosis. Cuboidal Epithelium. Cuboidal cells are roughly‚ a cuboidal in shape. Each cell has a spherical nucleus in the centre. Cuboidal epithelium is found in glands and in the lining of the kidney tubules as well as in the ducts of the
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