David Kennedy Bio 210 Lab Report 1 10/11/13 Microbial Growth Background Information: This lab was conducted in order to understand basic differences among differential and selective media‚ while recognizing how each media is used to isolate and identify microorganisms (Wistreich‚ 2003). The first microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive‚ single celled‚ arranged in grape-like clusters‚ and cocci in shape (Bukhari‚ 2004). S. epidermidis is approximately
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1 International Journal of Tropical Medicine and Public Health Volume 1‚ Issue 1‚ 2011 Crosshouse books Original Research Paper INVESTIGATING THE EFFECT OF A LOCAL HERB- P. AMARUS (SCHUM. AND THONN.) ON ENTEROPATHOGENIC STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI. BY ETTA‚ Hannah Edim‚ ELOMA‚ Nnanke‚ OKON‚ Essien Archibong and OFFOR‚ Ubana BIOLOGICAL SCIENCE DEPARTMENT‚ CROSS RIVER UNIVERSITY OF TECHNOLOGY ‚ CALABAR‚ CROSS RIVER STATE‚ NIGERIA. Abstract Objectives: Entero-pathogenic Staphylococcus
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groups then placed in agar plates simulating different environments: the bacteria lacking the pGLO plasmid was subjected to an environment solely contaiing nutrients and another containing nutrients and ampicillin; the bacteria containing pGLO was subjected to an environment containing solely nutrients and one containing nutrients‚ ampicillin‚ and the sugar arabinose. After being allowed to grow in their respective environments‚ the following agar plates grew E. Coli colonies: agar containing nutrients
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Introduction: Microbes‚ also called microorganisms‚ are minutes living things that individually are usually too small to be seen with the unaided eye. The group includes bacteria‚ fungi (yeast and molds)‚ protozoa and microscopic algae. It also includes viruses‚ those noncellular entities sometimes regarded as straddling the border between life and nonlife. People tend to related these microbes only with major disease such as AIDS‚ uncomfortable infections‚ or such common inconveniences as spoiled
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Course: BIO-205 BD2 Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated‚ why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time‚ propose is to isolate the unknown bacteria. Therefore‚ the first time to streak on the plate‚ there are million of bacteria on the
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002141-0012 Nuba Jackson IB Biology Microbiology IA How effective is Lysol in the reduction of bacterial growth compared to Pinesol in reduction of E. Coli growth in agar at room temperature? Background Information: Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99.9 percent of bacteria. Lysol contains
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Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB Agar (pg 170)‚ Mannitol Salt Agar (MSA)(pg 172) )‚ MacConkey Agar (pg 174)‚ and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to lyse red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them?
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fight‚ and would stop its growth. HA- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Penicillin and the B. subtilis will grow more around the Tetracycline‚ because E. coli is resistant to Penicillin and B. subtilis is resistant to Tetracycline. HO- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Tetracycline and the B. subtilis
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“Super clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed
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suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in a water bath 5 tubes of 9.0 ml trypticase soy (TS) broth 1 ml sterile pipettes Pipette aids Methods: 1. We began the experiment by marking the plates
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