Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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For this experiment you will need 39 agar plates‚ sterile cotton swabs‚ a concentrated amount of Escherichia coli (E. coli)‚ three different levels of antibiotic (Ampicillin: 1mg‚ 5mg‚ and 10mg)‚ a bunsen burner‚ metal tweezers‚ an incubator‚ 27 broths‚ and a ruler which should all be provided through your laboratory. For the first generation‚ you will be using 12 plates. Three of the plates will be labeled G1 control‚ three of the plates will be labeled G1/1 mg‚ three plates will be labeled G1/5
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simple diffusion and understand how to make % solutions. Exercise 2 demonstrated the principle of osmosis: observing the movement of water by osmosis from an area of lower concentration of a solute to an area of higher concentration of a solute. Materials and Method: Refer to pages 47-51 of Bio 203L Lab Manual. Results/ Discussion: The effect of the chloride ion on its rate of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride
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Factors Affecting Diffusion How does temperature affect diffusion? What is Diffusion? Definition 1: Diffusion is a process where by molecules move from an area of high concentration area to an area of low concentration. Definition 2: Diffusion is an evidence for moving particles. The spreading out of a gas is called diffusion and it takes place in haphazard and random way. Gases diffuse because the particles collide with other particles and bounce of in all directions. For example when
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DIFFUSION REVIEW WORKSHEET 1. Define diffusion and what causes it to occur? The action of spreading the light from a light source evenly so as to reduce glare and harsh shadows. Diffusion is caused when entities are small and moving in molecules. They collide because they are moving at a random pace. The temperature causes diffusion. 2. Define the terms hypertonic‚ hypotonic‚ and isotonic solution. * Hypertonic: Having increased pressure or tone‚ in particular. * Hypotonic:
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The effect of molecular weight on the diffusion rates of Potassium permanganate (KMnO4)‚ Potassium dichromate(K2Cr2O7) and Methylene Blue^1 _________________ Group 3 Sec. X-1L February 6‚ 2012 ____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium
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Diffusion in Innovations Submitted to Dr. Darrell Whiteman in partial fulfillment of the requirements for the course ME 8000-9000 Missiological Anthropology and Cultural Change at Trinity Evangelical Divinity School Deerfield‚ Illinois October 2013 An innovation is “an idea‚ practice‚ or object that is perceived as new by an individual or another unit of adoption” (Rogers 306-3071). Is the Gospel an innovation or is it an ongoing active work
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1 Diffusion is the movement of molecules (or ions) from a high concentration to low concentration. Diffusion is form of passive transport as energy does not need to be generated. Diffusion is complete when the concentration of molecules is equal on either side of the membrane. Diffusion rate can be influenced by many factors such as: Concentration gradient across the the membrane. Permeability of the membrane to the diffusing substance. Temperature. Surface area of the membrane. Question 2 2.1
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‘Agar extract as additive component in making floor wax’ Jericho Earl F. Follosco Clifford Cyril R. Jumawan Abul Ghaffar A Lintasan (Researchers) Dr. Estela Florentino (Researchers Teacher) B. Statement of the Problem This study aims to produce an agar extract as additive component in making floor wax. Specifically it sought to answer the following questions: a.) Which of the following concentrations
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