separate the polypeptides in the mixture and was visualized by coomassie brilliant blue and silver staining. Western blot method was used to detect the molecular weight of proteins which is selected for three different antibodies: α-β-actin targeting the α and β isoforms of actin protein‚ α-GADPH targeting Glyceraldehyde 3-phosphate dehydrogenase‚ (GAPDH); and α-BSA which targets bovine liver protein. Target protein was identified with TANDEM mass spectrometry. The experiment can be labeled a success since
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Skeletal System Skeletal system is the biological system providing support in living organisms. Skin‚ muscle and bones allow movement. Skin - pliable covering. Muscles do actual moving. Bones give anchor to move against. The skeleton functions not only as the support for the body but also in haematopoiesis‚ the manufacture of blood cells that takes place in bone marrow. This is why people who have cancer of the bone marrow almost always die. It is also necessary for protection of vital organs
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INTRODUCTION A major obstacle in the successful treatment of cancer is the development of resistance mechanisms to drug treatment. Various cellular changes that have been implicated in the development of drug resistance in cancer cells include: the increased expressions of P-glycoprotein (a multidrug transport protein) and multidrug resistance-associated protein (MRP-1); increased levels of the cellular detoxification protein‚ glutathione; and changes in the expression of apoptosis associated
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The Muscular System: Neuromuscular Junction 1. What insulates each muscle cell? __________endomysium_______________ 2. Synaptic vesicles in the axon terminal of a motor neuron contain what neurotransmitter? __________acetylcholine_______________ 3. An action potential in the axon terminal of a motor neuron opens what type of ion channels? ____________Voltage-gated Calcium_____________ 4. By what means of membrane transport does the neurotransmitter leave the axon
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Chapter 10- Muscle Tissue Choose the best answer to each of the following questions. 1) How does muscle tissue contribute to homeostasis? A) by generating heat that helps maintain body temperature B) by moving materials through the body C) by pulling on bones to move the body D) A and B are correct. E) A‚ B and C are correct. 2) The primary function of muscle is A) conversion of chemical energy into mechanical energy. B) conversion of chemical energy into radiant energy. C) conversion
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A Tour of the Cell Overview: The Importance of Cells • All organisms are made of cells. o Many organisms are single-celled. o Even in multicellular organisms‚ the cell is the basic unit of structure and function. • The cell is the simplest collection of matter that can live. • All cells are related by their descent from earlier cells. Concept 2 Eukaryotic cells have internal membranes that compartmentalize their functions Prokaryotic and eukaryotic cells
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Erythrocytes Survive 120 Days Hemoglobin * Globin: 4 folded polypeptide chains * Heme: Iron group in each polypeptide that binds 02 Enzymes: * Glycolytic: generate energy through glycolysis due to lack of mitochondria * Carbonic anhydrase: for transport of CO2 into HCO3 Erythropoiesis: * regeneration of new cells in the bone marrow Erythropoietin (hormone): * when kidneys detect not enough 02 they secrete this hormone which stimulates erythropoiesis‚ increasing 02 capacity
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MITOSIS What is Mitosis? There are various structures within the cell‚ but many are too difficult to see. For example‚ within the nucleus lie the chromosomes. These are important for heredity and reproduction. When a cell splits and becomes two‚ certain processes occur within the nucleus first. One of these processes involves the splitting of the chromosomes. This process is called Mitosis and there are four distinct stages. Mitosis takes place in Regular Body Cells or Somatic Cell. It keeps
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NATIONAL CENTER FOR CASE STUDY TEACHING IN SC CE Wearing on Her Nerves: Exploring the Interrelation between the Nervous and Muscular Systems by Kathleen G. Brown‚ Nursing Department Sharon S. Ellerton‚ Biological Sciences and Geology Queensborough Community College‚ City University of New York Part I – Rise and Shine? Kathy‚ a 20-year-old woman‚ awakens one morning to a tingling‚ numb sensation covering both of her feet. This has happened to her a number of times throughout the year
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Muscle Tissue Introduction * Motion * Results from alternating contraction (shortening) and relaxation of muscles * Skeletal system * Provides leverage and a supportive framework for this movement * Myology – study of muscles Muscle Tissue * Alternating contraction and relaxation of cells * Chemical energy changed into mechanical energy Types of Muscle Tissue * Skeletal muscle – primarily attached to bones * Striated and voluntary * Cardiac
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