Isolation and Characterization of Maize Acid Phosphatase | BCHM 405 | Aaron BANESEH 10307275 | Table of Contents 1 Table of Contents 2 2 Introduction 4 3 Experimental Procedures 5 3.1 Sample preparation 5 3.2 Initial Extraction of Acid Phosphatase 5 3.3 Determination of Protein Concentration by the Folin-Lowry Assay 5 3.4 Specific Activity Assay 6 3.4.1 Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of
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concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction: Alkaline Phosphatase: Alkaline Phosphatase is an enzyme
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effect of citric and buffered lactic acids on the flavour of hard-boiled sweets and the effect of acids on various flavours in high temperature applications. Introduction Materials and Methods An amount of water‚ sugar and glucose syrup of 30g‚ 100g and 70g were weighed respectively into a stainless steel pot. The mixture was then heated and removed immediately from the induction cooker after reaching the desired temperature of 145˚C. Flavours of 0.51g and acid of 1.20g was added immediately afterwards
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substances. One way is based on their pH. Substances may be * Acid 2. Base 3. Neutral INDICATORS This is a substance which is one color in and acid and another color in a base. Indicators can ten be used to test for the presence of acids or bases in a substance. Some are available in paper form (litmus) and others in liquid form (methyl orange). INDICATOR | COLOR IN ACID | COLOR IN BASE | LITMUS |
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LAB 6. ACIDS AND BASES: PH AND BUFFERS PURPOSE: To determine the pH of common acids and bases using a pH meter‚ pH paper‚ and red cabbage indicator. To test the effect of adding an acid or base to a buffer solution. SAFETY CONCERNS: Always wear safety goggles. Wash with soap and water if skin contacts acids or bases. ACIDS: An Acid is a substance that when dissolved in water will produce hydrogen ions‚ H+‚ in the solution. An acid that does not contain carbon is called an inorganic
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Acid Property #1: The word acid comes from the Latin word acere‚ which means "sour." All acids taste sour. Well known from ancient times were vinegar‚ sour milk and lemon juice. Aspirin (scientific name: acetylsalicylic acid) tastes sour if you don’t swallow it fast enough. Other languages derive their word for acid from the meaning of sour. So‚ in France‚ we have acide. In Germany‚ we have säure from saure and in Russia‚ kislota from kisly. Base Property #1: The word "base" has a more complex
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Suggested time allotment: 5 to 6 hours MODULE 2 ACIDS AND BASES In Module 1‚ you identified common properties of solutions using different methods. You learned how to report the amount of the components in a given volume of solution. You also found out that not all solutions are liquid. Some of them are solids and others are gases. Towards the end of the module‚ you investigated the factors that affect how fast a solid dissolves in water. Most of the solutions you studied in Module 1
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Experiment 4: ACIDS AND BASES: PH Measurements and Macroscale Titration CHM023L – A12 Group no. 6 Members: | Contributions: | | Conclusion | | Recommendation | | Tables and figures with analysis | | Principles‚ Equation | | Abstract‚ tables | Date Performed: February 28‚ 2012 - Tuesday Date Submitted: March 6‚ 2012 - Tuesday Submitted to: ------------------------------------------------- ABSTRACT: This experiment introduces us the pH measurement and application of macroscale titration
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| An acid is a chemical substance any of various typically water soluble and sour compounds that are capable of reacting with a base to for a salt‚ that redden litmus paper‚ that evolve hydrogen on reaction with various metals‚ that in water solution yield hydrogen ions‚ and that have hydrogen containing molecules or ions able to give up a proton to a base or that are substances able to accept and unshared pair of electrons from a base. An alkaline is a chemical compound that neutralizes or effervesces
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What is Acid Rain? First identified in 1872 in Sweden and studied in the U.S. beginning in the 1950s‚ acid rain is precipitation in the form of rain‚ snow‚ hail‚ dew‚ or fog that transports sulfur and nitrogen compounds from the high atmosphere to the ground. Sulfur dioxide (SO2) and nitrogen oxides (NO‚ NO2) are bi-products from burning fuels in electric utilities and from other industrial and natural sources. These chemicals react with water‚ oxygen‚ carbon dioxide‚ and sunlight in the atmosphere
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