INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions: 1. Describe
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Plant Transport Plants are mostly made up of water. Lettuce is about 94% water and a potato is about 77% water. Plants need water for three main purposes: ------photosynthesis‚ -support -transport of chemicals Water is a raw material for photosynthesis. If water is in short supply‚ the rate of photosynthesis will be limited. Plants need water for support‚ otherwise they wilt. Water is needed to transport many chemicals within plants. Chemicals‚ such as mineral salts‚ dissolve in water and
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Lab 11- Plant pigment and Photosynthesis Zelda Salfati December 20th‚ 2012 BIOL 1012Y Partners Lab: Nicole Mintz‚ Bayla Kronman
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The Absorption Spectrum of Cobalt(II) Chloride Thang Dinh Lab partner: Alex Yang CHM 151 – 102 11/17/2014 Introduction: Many compounds absorb a certain spectrum of visible light. Since Beer’s Law describe the relationship between molar absorptivity and concentration‚ one could use his equation A = kc to find the unknown concentration by the known absorptivity. The graph of absorption spectrum is represented by a linear regression. From the graph‚ one could deduct that as the concentration decreases
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of this lab was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment in chloroplasts. The chemical
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Electromagnetic energy‚ in the form of light‚ travels in wavelengths and is measured in nanometres (nm). Pigments within matter‚ such as plants‚ absorb varying wavelengths of light. The absorption spectrum can be defined as a graphical representation of a pigment’s ability to absorb light against varying wavelengths (nm). The pigments light absorbing ability is measured using a spectrophotometer. An action spectrum can be defined as the graphical representation of the rate of photosynthesis against wavelengths
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chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they are not equally
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