Five-Layer Density Column A density column consists of layers of liquids of different densities which do not mix with each other‚ and which are clearly distinguishable from each other. The highest density material is at the bottom of the column‚ and as you proceed up the column‚ the density of each successive layer decreases‚ with the lowest density layer on top. Three methods for construction of a five-layer density column are discussed in this write-up. All methods use the same five liquids. •
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Density Column What is density? We will be working with many different liquids. Each of the liquids has a different‚ characteristic density. One way to think about density is how thick or heavy an object is. The liquids with lighter densities will float. The liquids with heavier densities will drop to the bottom. You may have noticed that sometimes‚ liquids mix together – this is because the chemicals dissolve in each other. During our experiment‚ the liquids will stay separate because we will
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=========== ORGANIC CHEMISTRY ============ Organic Chemistry Laboratory - CH 201 (2010-2011) Experiment 4B Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________
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COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS‚ Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO‚ John Michael; SABINO Patricia Anne; SANTOS‚ Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components‚ the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment‚ the solid used by the students was
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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The 7 Layers of the OSI Model Last updated: March 03‚ 2008 The OSI‚ or Open System Interconnection‚ model defines a networking framework for implementing protocols in seven layers. Control is passed from one layer to the next‚ starting at the application layer in one station and proceeding to the bottom layer‚ over the channel to the next station and back up the hierarchy. |Application |This layer supports application and end-user processes. Communication partners
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The Seven Layers of the OSI Model Kevin Ludwig Bryant and Stratton College TECH 140 Khaled Sabha 12.08.2009 There are seven layers in the OSI model. Each layer has a function or purpose that is used to set up a network. The International Organization for Standardization began to develop the OSI framework in 1984. The purpose was to have each layer interact with the layer directly beneath it. The layers are starting from the top: Application‚ Presentation‚ Session‚ Transport‚ Network
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as the stationary phase. After
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The 7 layers can be split logically into two subgroups. Layers 7 thru 4 focus on the end to end communication of data source and destinations. Layers 3 thru 1 are provide consistent communication between the network devices. An easier way of looking at the OSI model is dividing the upper layers (7‚ 6‚ 5) from the lower layers (4‚ 3‚ 2‚ 1). The upper layers deal with application issues and are implemented only in software. The highest layer‚ the application layer‚ is the closest to the end user
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