Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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I. Purpose The purpose of this lab is to determine the molecular mass of a volatile liquid. II. Procedure • Place a cork with a small hole in it inside of a test tube and find the weight of the two together using a sensitive balance. • Pour 0.5 mL of the unknown volatile liquid into the test tube‚ insert the cork‚ and place the tube into boiling hot water while keeping the cork above water level. As the gas evaporates‚ excess gas will be released throughout the whole in the cork. Keep the test
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the cloning of the gene are given below and underlined on the nucleic acid sequence in section “b”: Forward: CCGGAAGAGA GTCAATTCAG GGTGGTG Reverse: CACTCATTAG GCACCCCAGG i. The vector system chosen for the gene is the pBAD/HIS vector. See appendix 3 for an image of this vector. The chosen primers with the attached restriction site are sequences (in bold) are: Forward: (XhoI) GGGCTCGAG CCGGAAGAGA GTCAATTCAG GGTGGTG Reverse: (Bgl II) GGGAGATCT CACTCATTAG GCACCCCAGG ii. The primers chosen follow
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1. Atomic and Molecular Structure a. Students know how to relate the position of an element in the periodic table to its atomic number and atomic mass. The Periodic Table organizes elements by their atomic number - from hydrogen (1) to whatever is the highest one currently known (>105). It is arranged so that similiar members fall in a list such as Chlorine Bromine etc.. The average atomic weight is usually shown with each element‚ but due to isotopes (caused buy nuclear varations)
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WHAT IS TORQUE? Torque is a measure of how much a force acting on an object causes that object to rotate. The object rotates about an axis‚ which we will call the pivot point‚ and will label ’O ’. We will call the force ’F ’. The distance from the pivot point to the point where the force acts is called the moment arm‚ and is denoted by ’r ’. Note that this distance‚ ’r ’‚ is also a vector‚ and points from the axis of rotation to the point where the force acts. (Refer to Figure 1 for a pictoral
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Kinetics of an SN1 reaction: the effect of solvent on rate Object The purpose of this experiment is to determine the rate of hydrolysis in acetone/water (50/50 v/v and 60/40 v/v). Background and Theory An SN1 reaction of tert-butyl chloride takes place in two steps. First‚ the Alkyl Halide will leave the molecule. In this step the bond is breaking‚ which takes a longer amount of time‚ so it will determine the rate of the reaction. As a result‚ it forms a tertiary carbocation‚ since this
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The two different types of energy are kinetic and potential energy. Kinetic energy is the energy a moving object has because of its motion. The kinetic energy of a moving object depends on the object’s mass and its speed. The kinetic energy of a moving object can be calculated from this equation: Kinetic energy (in joules) = ½ mass (in kg) x [speed (in m/s)]² KE= ½ mv² In this equation ^‚ the symbol v represents speed. Example Find the kinetic energy of the ball having mass 0‚5 kg and
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Ultra thin film coatings can be used in a wide range of applications as lubricants‚ anti-stiction layers‚ molecular glues‚ coupling agents or adhesion layers. The mentioned molecular coatings are used to modify material surface properties. The traditional coating deposition methods consist of a set of sequential wet baths. The wet processing method is complex and limits the ability of molecular coating in many MEMS applications. The use of liquid precursors has extremely limited bath life‚ which cause
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Static and Kinetic Friction Introduction The amount of friction force between two surfaces in contact depends on the type of the surfaces in contact and the amount of compression between the surfaces. Static friction is the force that is acting against your force before the object begins to move. If you exert a small push on the box‚ the box will not move because static friction is directly opposite to the push. If you apply a greater force than the static friction force‚ the friction increases
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Empirical and Molecular Formula | Key Concepts * Empirical Formula of a compound shows the ratio of elements present in a compound. * Molecular Formula of a compound shows how many atoms of each element are present in a molecule of the compound. * The empirical formula mass of a compound refers to the sum of the atomic masses of the elements present in the empirical formula. * The Molecular Mass (formula mass‚ formula weight or molecular weight) of a compound is a multiple of the empirical
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