Swag
Abstract:
This lab works to develop the understanding of bacterial transformation through the integration of a plasmid into E-coli bacteria. Understanding of plasmids, GFP, expression of genes and bacterial candidates is used to formulate a lab which demonstrates a variety of factors associated with transformation efficiency. It was deduced that there are certain requirements present in the pGLO plasmid for full gene expression and that an increase in transformation solution positively impacts the results with an increase in growth.
Introduction:
The pGLO lab is a lab that uses the theory of bacterial transformation to undergo its procedure. Bacterial Transformation is the insertion of foreign DNA into a cell thereby altering its DNA. When the gene is introduced (pGLO), the genetic information is absorbed into the plasmid of the bacterium. The DNA is then coded as if it were part of the original DNA. This new DNA is classified as recombinant DNA. The method used in this lab for bacterial transformation is a combination of a calcium chloride solution followed by a heat shock. The heat shock is then used to increase the permeability of the membranes present in the bacteria which allows for the easy introduction of the foreign DNA. Fredrick Griffith was the first person to attempt a bacterial transformation lab in 1928. He combined a pathogenic and non-pathogenic strand of pneumococcus bacteria that was treated with heat, he was able to develop a mixture that contained no virulent bacteria yet killed all of the subjects it was tested on. This was a great breakthrough in the field of biotechnology. The bacteria chosen in this experiment is E-coli. Escherichia coli has become a "model organism" for studying many of life's essential processes. Due to its rapid growth rate, simple nutritional requirements, well established genetics and completed genomic sequence, more is now known about E. coli than any other living organism. It’s ability to reproduce so quickly
This lab works to develop the understanding of bacterial transformation through the integration of a plasmid into E-coli bacteria. Understanding of plasmids, GFP, expression of genes and bacterial candidates is used to formulate a lab which demonstrates a variety of factors associated with transformation efficiency. It was deduced that there are certain requirements present in the pGLO plasmid for full gene expression and that an increase in transformation solution positively impacts the results with an increase in growth.
Introduction:
The pGLO lab is a lab that uses the theory of bacterial transformation to undergo its procedure. Bacterial Transformation is the insertion of foreign DNA into a cell thereby altering its DNA. When the gene is introduced (pGLO), the genetic information is absorbed into the plasmid of the bacterium. The DNA is then coded as if it were part of the original DNA. This new DNA is classified as recombinant DNA. The method used in this lab for bacterial transformation is a combination of a calcium chloride solution followed by a heat shock. The heat shock is then used to increase the permeability of the membranes present in the bacteria which allows for the easy introduction of the foreign DNA. Fredrick Griffith was the first person to attempt a bacterial transformation lab in 1928. He combined a pathogenic and non-pathogenic strand of pneumococcus bacteria that was treated with heat, he was able to develop a mixture that contained no virulent bacteria yet killed all of the subjects it was tested on. This was a great breakthrough in the field of biotechnology. The bacteria chosen in this experiment is E-coli. Escherichia coli has become a "model organism" for studying many of life's essential processes. Due to its rapid growth rate, simple nutritional requirements, well established genetics and completed genomic sequence, more is now known about E. coli than any other living organism. It’s ability to reproduce so quickly